Ksandopulo G B, Ruban E L
Biol Bull Acad Sci USSR. 1979 Mar-Apr;6(2):261-4.
The optimal conditions for the manifestation of the lipase activity of an enzyme preparation from the culture liquid of the fungus Geotrichum asteroides were determined. It was found that the rate of hydrolysis depends on the substrate concentration, the enzyme concentration, and the reaction time. The lipase from G. asteroids is rather stable--it loses practically no activity in a year at 4 degrees C. The temperature optimum of the enzyme preparation is 37 degrees. The lipase from G. asteroides is most active at pH 8.0; it retains its activity when kept for 3 h in a reaction medium with a pH from 3 to 10.
确定了来自星状地霉培养液的酶制剂中脂肪酶活性表现的最佳条件。发现水解速率取决于底物浓度、酶浓度和反应时间。星状地霉的脂肪酶相当稳定——在4℃下一年几乎不丧失活性。该酶制剂的最适温度为37℃。星状地霉的脂肪酶在pH 8.0时活性最高；当在pH值为3至10的反应介质中保持3小时时，它仍保留其活性。
