Zhu Xiaopei, Chai Min, Li Yang, Sun Meiyu, Zhang Jinzheng, Sun Guofeng, Jiang Chuangdao, Shi Lei
Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of SciencesBeijing, China; University of Chinese Academy of SciencesBeijing, China.
Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences Beijing, China.
Front Plant Sci. 2016 Apr 19;7:491. doi: 10.3389/fpls.2016.00491. eCollection 2016.
As a popular ornamental flower, potted lily is an important object of lily breeding. Paclobutrazol, a chemical growth retardation compound, is often used to dwarf plant in producing potted lilies. However, in recent years, the plants with inherited dwarf traits by using genetic engineer breeding technology are being developed. The studies on molecular basis of lily dwarfism will offer some target genes which have profound dwarf effect for genetic engineer breeding. Here, we confirmed that paclobutrazol inhibited plant height and leaf size in Lilium Longiflorum-Asiatic hybrid, and then RNA-Seq technique was employed to analyze gene transcripts of Lilium Longiflorum-Asiatic hybrid leaves by paclobutrazol treatment in order to get a deeper insight into dwarfism mechanism of lily. Approximately 38.6 Gb data was obtained and assemble into 53,681 unigenes. Annotation, pathways, functional classification and phylogenetic classification of these data were analyzed based on Nr, Nt, Swiss-Prot, KEGG, COG, and GO databases. 2704 differentially expressed genes were screened by comparing paclobutrazol-treated samples with untreated samples and quantitative real-time PCR was performed to validate expression profiles. By analyzing dynamic changes of differentially expressed genes, nine metabolic pathways and signal transduction pathways were significantly enriched and many potentially interesting genes were identified that encoded putative regulators or key components of cell division, cell expansion, GA metabolism and signaling transduction and these genes were highlighted to reveal their importance in regulation of plant size. These results will provide a better understanding of the molecular mechanism on lily dwarfism and some potential genes related to lily organ size, which will lay the foundation for molecular breeding of potted lilies. These transcriptome data will also serve as valuable public genomic resources for other genetic research in lily.
作为一种受欢迎的观赏花卉，盆栽百合是百合育种的重要对象。多效唑是一种化学生长延缓化合物，在盆栽百合生产中常用于使植株矮化。然而，近年来，利用基因工程育种技术培育具有遗传性矮化性状植株的工作正在开展。对百合矮化分子基础的研究将为基因工程育种提供一些具有显著矮化效应的靶基因。在此，我们证实多效唑抑制了亚洲百合杂种系的株高和叶片大小，随后采用RNA测序技术分析多效唑处理的亚洲百合杂种系叶片的基因转录本，以更深入了解百合的矮化机制。获得了约38.6 Gb的数据，并组装成53,681个单基因。基于Nr、Nt、Swiss-Prot、KEGG、COG和GO数据库对这些数据进行注释、通路、功能分类和系统发育分类。通过比较多效唑处理样品和未处理样品，筛选出2704个差异表达基因，并进行实时定量PCR验证表达谱。通过分析差异表达基因的动态变化，9条代谢途径和信号转导途径显著富集，鉴定出许多潜在有趣的基因，这些基因编码细胞分裂、细胞扩张、赤霉素代谢和信号转导的假定调节因子或关键组分，强调这些基因在调节植株大小中的重要性。这些结果将有助于更好地理解百合矮化的分子机制以及一些与百合器官大小相关的潜在基因，为盆栽百合的分子育种奠定基础。这些转录组数据也将作为百合其他遗传研究的宝贵公共基因组资源。
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