PtrDUF579-3表达的抑制导致杨树中葡萄糖醛酸木聚糖的结构变化。
Suppression of PtrDUF579-3 Expression Causes Structural Changes of the Glucuronoxylan in Populus.
作者信息
Song Dongliang, Gui Jinshan, Liu Chenchen, Sun Jiayan, Li Laigeng
机构信息
National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology - Chinese Academy of Sciences Shanghai, China.
出版信息
Front Plant Sci. 2016 Apr 11;7:493. doi: 10.3389/fpls.2016.00493. eCollection 2016.
DUF579 (domain unknown function 579) genes have been reported to play diverse roles in cell wall biosynthesis, such as in glucuronoxylan (GX) synthesis. As GX is a major type of hemicelluloses in hard wood species, how DUF579 genes function in wood formation remains to be demonstrated in planta. This study reports a Populus DUF579 gene, PtrDUF579-3, which is characterized for its function in wood cell wall formation. PtrDUF579-3 is localized in Golgi apparatus and catalyzes methylation of the glucuronic acid (GlcA) in GX biosynthesis. Suppression of PtrDUF579-3 expression in Populus caused a reduction in both the GlcA side chain number and GlcA side chain methylation on the GX backbone. The modified GX polymer through PtrDUF579-3 suppression was more susceptible to acid treatment and the PtrDUF579-3 suppressed plants displayed enhanced cellulose digestibility. These results suggest that PtrDUF579-3 is involved in GX biosynthesis and GX structure can be modified through PtrDUF579-3 suppression in Populus.
据报道，DUF579（功能未知结构域579）基因在细胞壁生物合成中发挥多种作用，例如在葡糖醛酸木聚糖（GX）合成中。由于GX是硬木树种中半纤维素的主要类型，DUF579基因在木材形成中的功能仍有待在植物中证实。本研究报道了一个杨树DUF579基因PtrDUF579 - 3，其特点是在木材细胞壁形成中发挥作用。PtrDUF579 - 3定位于高尔基体，催化GX生物合成中葡萄糖醛酸（GlcA）的甲基化。抑制杨树中PtrDUF579 - 3的表达导致GX主链上GlcA侧链数量和GlcA侧链甲基化均减少。通过抑制PtrDUF579 - 3修饰的GX聚合物对酸处理更敏感，并且抑制PtrDUF579 - 3的植物表现出增强的纤维素消化率。这些结果表明PtrDUF579 - 3参与GX生物合成，并且可以通过抑制杨树中的PtrDUF579 - 3来修饰GX结构。
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