伊朗人群中白细胞介素-10基因启动子多态性（-592 A/C、-819 T/C、-1082 A/G）与乙肝病毒感染易感性的关联
Association Between IL-10 Gene Promoter Polymorphisms (-592 A/C, -819 T/C, -1082 A/G) and Susceptibility to HBV Infection in an Iranian Population.
作者信息
Moudi Bita, Heidari Zahra, Mahmoudzadeh-Sagheb Hamidreza, Hashemi Mohammad, Metanat Malihe, Khosravi Soheila, Farrokh Parisa
机构信息
Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran; Department of Histology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IR Iran.
Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IR Iran.
出版信息
Hepat Mon. 2016 Feb 20;16(2):e32427. doi: 10.5812/hepatmon.32427. eCollection 2016 Feb.
BACKGROUND
IL-10 can play a vital role in immune response against HBV. Three biallelic SNPs from the transcription start site control the transcription of the IL-10 gene. An association between susceptibility to HBV and IL-10 polymorphisms has been suggested in patients with HBV infection.
OBJECTIVES
The present study was designed to study the association between polymorphisms in interleukin-10 (-1082 A/G, -819 T/C and -592 A/C) promoter gene and chronic hepatitis B virus (HBV) infection.
PATIENTS AND METHODS
221 chronically infected patients and 200 healthy control subjects were enrolled in the study. Three biallelic (-1082 A/G, -819 T/C and -592 A/C) polymorphisms in the IL-10 promoter gene were determined by PCR-RFLP method.
RESULTS
Persistent HBV infection was associated with IL-10-1082 AG (P = 0.001) and GG (P = 0.004) genotypes and G (P = 0.000) allele. IL-10-819 T/C and -592 A/C genotype and allele frequencies did not show any correlation with the risk of chronic hepatitis B infection.
CONCLUSIONS
These results suggest that polymorphisms in interleukin-10 gene promoter influence clinical outcome of HBV infection and susceptibility to HBV infection.
背景
白细胞介素-10（IL-10）在针对乙肝病毒（HBV）的免疫反应中发挥着至关重要的作用。来自转录起始位点的三个双等位基因单核苷酸多态性（SNP）控制着IL-10基因的转录。HBV感染患者中，HBV易感性与IL-10基因多态性之间的关联已被提出。
目的
本研究旨在探讨白细胞介素-10（-1082 A/G、-819 T/C和-592 A/C）启动子基因多态性与慢性乙肝病毒（HBV）感染之间的关联。
患者和方法
本研究纳入了221例慢性感染患者和200例健康对照者。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法测定IL-10启动子基因的三个双等位基因（-1082 A/G、-819 T/C和-592 A/C）多态性。
结果
持续性HBV感染与IL-10 -1082 AG（P = 0.001）和GG（P = 0.004）基因型以及G（P = 0.000）等位基因相关。IL-10 -819 T/C和-592 A/C基因型及等位基因频率与慢性乙肝感染风险未显示出任何相关性。
结论
这些结果表明，白细胞介素-10基因启动子多态性影响HBV感染的临床结局及对HBV感染的易感性。
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