Roux-en-Y胃旁路手术对肠道嗜黏蛋白阿克曼氏菌的影响。
Effect of Roux-en-Y gastric bypass surgery on intestinal Akkermansia muciniphila.
作者信息
Yan Ming, Song Mao-Min, Bai Ri-Xing, Cheng Shi, Yan Wen-Mao
机构信息
Ming Yan, Mao-Min Song, Ri-Xing Bai, Shi Cheng, Wen-Mao Yan, Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
出版信息
World J Gastrointest Surg. 2016 Apr 27;8(4):301-7. doi: 10.4240/wjgs.v8.i4.301.
AIM
To investigated changes in intestinal Akkermansia muciniphila (A. muciniphila) and explored the mechanism underlying the therapeutic effects of Roux-en-Y gastric bypass (RYGB) surgery on type 2 diabetes in diabetic Goto-Kakizaki (GK) rats.
METHODS
Male diabetic GK rats (n = 12) aged 8 wk were randomly assigned to the surgery group (GK-RYGB) or sham surgery group (GK-Sham) (n = 6 per group), and another 6 male Wistar rats aged 8 wk served as controls (WS-Sham). In the surgery group, RYGB surgery was conducted, and a sham operation was performed in both sham groups. Fasting blood glucose (FBG) levels before and after surgery, fasting levels of serum insulin and serum glucagon-like peptide-1 (GLP-1) and levels 30 min after intragastric injection of glucose, and the amount of A. muciniphila in the stool were determined. Insulin and GLP-1 were measured by enzyme-linked immunosorbent assay, and A. muciniphila were detected by fluorescence-based quantitative polymerase chain reaction.
RESULTS
The FBG was improved, and serum GLP-1 and insulin increased significantly (P < 0.05) in the GK-RYGB group after surgery compared to levels before surgery and to levels in the GK-Sham group. Before surgery, the amounts of A. muciniphila in the GK-RYGB and GK-Sham groups were significantly lower than in the WS-Sham group (P < 0.05). After surgery, the amount of A. muciniphila in the GK-RYGB group increased markedly compared to that before surgery and to that in the GK-Sham and WS-Sham groups (P < 0.05). In addition, the A. muciniphila amount was positively related to GLP-1 (r = 0.86, P < 0.05).
CONCLUSION
Our results demonstrated RYGB surgery may increase GLP-1 secretion, elevate serum insulin after intragastric injection of glucose, and improve insulin resistance in diabetic GK rats, thereby contributing to a significant reduction in blood glucose. The increased amount of A. muciniphila after RYGB surgery may be related to elevated GLP-1 secretion.
目的
研究肠道嗜黏蛋白阿克曼氏菌（A. muciniphila）的变化，并探讨Roux-en-Y胃旁路术（RYGB）对糖尿病Goto-Kakizaki（GK）大鼠2型糖尿病治疗作用的潜在机制。
方法
将12只8周龄的雄性糖尿病GK大鼠随机分为手术组（GK-RYGB）或假手术组（GK-Sham）（每组6只），另取6只8周龄的雄性Wistar大鼠作为对照组（WS-Sham）。手术组进行RYGB手术，两个假手术组均进行假手术。测定手术前后的空腹血糖（FBG）水平、空腹血清胰岛素和血清胰高血糖素样肽-1（GLP-1）水平以及胃内注射葡萄糖30分钟后的水平，同时测定粪便中嗜黏蛋白阿克曼氏菌的数量。采用酶联免疫吸附测定法检测胰岛素和GLP-1，采用荧光定量聚合酶链反应检测嗜黏蛋白阿克曼氏菌。
结果
与手术前及GK-Sham组相比，GK-RYGB组术后FBG改善，血清GLP-1和胰岛素显著升高（P<0.05）。术前，GK-RYGB组和GK-Sham组的嗜黏蛋白阿克曼氏菌数量显著低于WS-Sham组（P<0.05）。术后，GK-RYGB组的嗜黏蛋白阿克曼氏菌数量与术前及GK-Sham组和WS-Sham组相比显著增加（P<0.05）。此外，嗜黏蛋白阿克曼氏菌数量与GLP-1呈正相关（r=0.86，P<0.05）。
结论
我们的结果表明，RYGB手术可能增加GLP-1分泌，提高胃内注射葡萄糖后的血清胰岛素水平，并改善糖尿病GK大鼠的胰岛素抵抗，从而导致血糖显著降低。RYGB手术后嗜黏蛋白阿克曼氏菌数量的增加可能与GLP-1分泌升高有关。
Zotero 插件