Chen T M, Coutant J E
Merrell Dow Research Institute, Analytical Chemistry Department, Cincinnati, OH 45215-6300.
J Chromatogr. 1989 Jan 20;463(1):95-106. doi: 10.1016/s0021-9673(01)84456-3.
A method for the routine analysis of phenylthiocarbamyl (PTC) amino acids by thermospray high-performance liquid chromatography-mass spectrometry (TSP LC-MS) is described. Data were acquired on a small dedicated TSP LC-MS system in which the temperature of the vaporizer and ion source block were optimized. PTC-amino acids exhibited unique TSP mass spectra containing sufficient fragment ions to determine structural data. Therefore, using this method the amino acids contained in the acid hydrolysates of unique and modified peptides were able to be positively identified. Additionally, the amino acid composition of peptides as determined by TSP LC-MS in the selected ion monitoring mode corresponded well with the theoretical value. The detection limits for the PTC-amino acids were at the low picomole level.
本文描述了一种采用热喷雾高效液相色谱 - 质谱联用（TSP LC-MS）对苯硫代氨基甲酰（PTC）氨基酸进行常规分析的方法。数据采集于一个小型专用TSP LC-MS系统，该系统对蒸发器和离子源模块的温度进行了优化。PTC氨基酸呈现出独特的热喷雾质谱图，其中包含足够的碎片离子以确定结构数据。因此，使用该方法能够对独特肽段和修饰肽段的酸水解产物中所含的氨基酸进行阳性鉴定。此外，在选择离子监测模式下通过TSP LC-MS测定的肽段氨基酸组成与理论值吻合良好。PTC氨基酸的检测限处于低皮摩尔水平。
