Systemic Physiological and Ecotoxicological Research (SPHERE), Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerpen, Belgium; Integrated Molecular Plant Physiology Research (IMPRES), Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerpen, Belgium.
Zebrafishlab, Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgium.
Water Res. 2016 Aug 1;99:129-139. doi: 10.1016/j.watres.2016.04.059. Epub 2016 Apr 27.
Because of the ever increasing complexity of environmental contamination profiles, there are limitations to the use of analytical pollutant measurements for monitoring and prioritization of watercourses. The potential of biomarkers has been debated for many years, especially in laboratory settings, but there is a need for studies evaluating these approaches in the field. We evaluated the usefulness of a selection of biomarkers, mostly indicators of general physiological status and common stress responses such as oxidative stress, to discriminate among environmental pollution profiles, with the aim of prioritizing contaminated watercourses for targeted remediation efforts. To this end, juvenile common carp (Cyprinus carpio Lin.) were exposed in cages in the field to Flemish watercourses with varying pollution profiles. After six weeks of exposure, the bioaccumulation of key pollutants was measured, and a set of organismal, biochemical and transcriptional endpoints was determined in several tissue types. After data integration a discrete set of 14 parameters was identified, that could successfully distinguish all watercourses from each other. We show that an integrated biomarker approach, mainly targeting common stress responses, can offer the resolving power to discriminate among environmentally relevant exposure scenarios, and a means to prioritize watercourses for targeted remediation.
由于环境污染状况的日益复杂，分析污染物测量在监测和优先排序水道方面存在局限性。生物标志物的潜力多年来一直存在争议，特别是在实验室环境中，但需要在现场评估这些方法的研究。我们评估了一系列生物标志物的有用性，这些生物标志物主要是一般生理状态和常见应激反应（如氧化应激）的指标，以区分环境污染状况，旨在为有针对性的修复工作优先考虑受污染的水道。为此，我们将幼年鲤鱼（Cyprinus carpio Lin.）在野外的笼子中暴露于具有不同污染状况的佛兰芒水道。暴露六周后，测量了关键污染物的生物累积量，并在几种组织类型中确定了一组机体、生化和转录终点。在数据集成后，确定了一组离散的 14 个参数，可以成功地区分所有水道。我们表明，一种综合的生物标志物方法，主要针对常见的应激反应，可以提供区分与环境相关的暴露情况的分辨率，并为有针对性的修复优先考虑水道。
