Verrastro Maria, Cicco Nunzia, Crispo Fabiana, Morone Antonio, Dinescu Maria, Dumitru Marius, Favati Fabio, Centonze Diego
Scuola di Scienze Agrarie, Forestali, Alimentari ed Ambientali, Università degli Studi della Basilicata, Viale dell'Ateneo Lucano 10, 85100 Potenza, Italy; Istituto di Struttura della Materia, Consiglio Nazionale delle Ricerche, c/da S. Loja, 85050 Tito Scalo (PZ), Italy.
Istituto di Metodologie per l'Analisi Ambientale, Consiglio Nazionale delle Ricerche, c/da S. Loja, 85050 Tito Scalo (PZ), Italy.
Talanta. 2016 Jul 1;154:438-45. doi: 10.1016/j.talanta.2016.03.072. Epub 2016 Mar 24.
A Laccase-based biosensor for the determination of phenolic compounds was developed by using Matrix Assisted Pulsed Laser Evaporation as an innovative enzyme immobilization technique. and the deriving biosensor was characterized and applied for the first time. Laccase was immobilized onto different substrates including screen printed carbon electrodes and spectroscopic, morphologic and electrochemical characterizations were carried out. A linear range from 1 to 60μM was achieved working at 5.5pH and -0.2V detection potential vs Ag pseudoreference. The limits of detection and quantification were found to be 1 and 5μM, respectively. A good fabrication reproducibility, stability of response and selectivity toward interferents were also found The potential of the developed biosensor was tested in the determination of total polyphenol content in real matrices (tea infusion, ethanolic extract from Muscari comosum bulbs and aqueous solution of a food supplement from black radish root and artichoke leaves) and the results were compared with those obtained by using the Folin-Ciocalteu method.
通过使用基质辅助脉冲激光蒸发作为一种创新的酶固定技术，开发了一种用于测定酚类化合物的基于漆酶的生物传感器，并首次对所得生物传感器进行了表征和应用。将漆酶固定在包括丝网印刷碳电极在内的不同基质上，并进行了光谱、形态和电化学表征。在5.5pH和相对于银伪参比电极-0.2V的检测电位下工作，实现了1至60μM的线性范围。检测限和定量限分别为1和5μM。还发现该生物传感器具有良好的制备重现性、响应稳定性和对干扰物的选择性。在实际基质（茶浸液、葡萄风信子鳞茎乙醇提取物以及黑萝卜根和洋蓟叶食品补充剂水溶液）中总多酚含量的测定中测试了所开发生物传感器的潜力，并将结果与使用福林-西奥尔特法获得的结果进行了比较。
