Castrovilli Mattea Carmen, Bolognesi Paola, Chiarinelli Jacopo, Avaldi Lorenzo, Cartoni Antonella, Calandra Pietro, Tempesta Emanuela, Giardi Maria Teresa, Antonacci Amina, Arduini Fabiana, Scognamiglio Viviana
Institute of Structure of Matter, National Research Council, Area Della Ricerca di Roma 1, Via Salaria Km 29.300, 00015, Monterotondo, Italy.
Department of Chemistry, Sapienza University, Roma, Italy.
Biosens Bioelectron. 2020 Sep 1;163:112299. doi: 10.1016/j.bios.2020.112299. Epub 2020 May 16.
Enzymes immobilisation represents a critical issue in the design of biosensors to achieve standardization as well as suitable analytical performances in terms of sensitivity, selectivity, and stability. In this work electrospray deposition (ESD) has been exploited as a novel technique for the immobilisation of laccase enzyme on carbon black modified screen-printed electrodes. The aim is to fabricate an amperometric biosensor for phenolic compound detection. The electrodes produced by ESD have been analysed by scanning electron microscopy and characterised electrochemically to prove that this immobilisation technique is suited to manufacture high performance biosensors. The results show that the laccase enzyme maintains its activity after undergoing the electrospray ionisation process and deposition and the fabricated biosensor has improved performances in terms of storage (up to 3 months at room temperature) and working (up to 25 measurements on the same electrode) stability. The laccase-based biosensor has been tested for phenolic compound detection, with catechol as target analyte, in the linear range 2.5-50 μM, with 2.0 μM limit of detection, without interference from lead, cadmium, atrazine, and paraoxon, and without matrix effect in drinking, surface, and wastewater.
酶固定化是生物传感器设计中的一个关键问题，旨在实现标准化以及在灵敏度、选择性和稳定性方面具备合适的分析性能。在本研究中，电喷雾沉积（ESD）被用作一种将漆酶固定在炭黑修饰丝网印刷电极上的新技术。目的是制造一种用于检测酚类化合物的安培型生物传感器。通过扫描电子显微镜对ESD制备的电极进行了分析，并进行了电化学表征，以证明这种固定化技术适用于制造高性能生物传感器。结果表明，漆酶在经历电喷雾电离过程和沉积后仍保持其活性，并且所制备的生物传感器在储存（室温下长达3个月）和工作（同一电极上多达25次测量）稳定性方面具有改进的性能。基于漆酶的生物传感器已用于检测酚类化合物，以邻苯二酚作为目标分析物，线性范围为2.5 - 50 μM，检测限为2.0 μM，不受铅、镉、阿特拉津和对氧磷的干扰，并且在饮用水、地表水和废水中无基质效应。
