Department of Forensic Medicine, Medical University of Gdańsk, Gdańsk, Poland.
Department of Psychiatry, Otto-von-Guericke-University, Magdeburg, Germany.
Psychiatry Res. 2016 Jul 30;241:43-6. doi: 10.1016/j.psychres.2016.04.079. Epub 2016 Apr 27.
The dorsal raphe nucleus (DRN) is the main source of serotonergic innervation of forebrain limbic structures disturbed in suicidal behaviour. We have evaluated the transcriptional activity of ribosomal DNA (rDNA) in DRN neurons by AgNOR silver staining method. The cohort (containing 24 suicidal and 20 non-suicidal patients, and 28 controls) was previously analysed regarding diagnosis-related differences between schizophrenia and affective disorders. Significant decreases in both AgNOR and nuclear areas suggestive of attenuated rDNA activity were currently found in suicidal versus non-suicidal patients. This effect, which was more accentuated in affective disorders patients, was not explained by antidepressant and antipsychotic medication.
中缝背核（DRN）是扰乱自杀行为的大脑边缘结构中 5-羟色胺能神经支配的主要来源。我们通过 AgNOR 银染法评估了 DRN 神经元中核糖体 DNA（rDNA）的转录活性。该队列（包含 24 名自杀和 20 名非自杀患者以及 28 名对照者）之前根据精神分裂症和情感障碍之间的诊断相关差异进行了分析。与非自杀患者相比，目前在自杀患者中发现 AgNOR 和核面积均显著减少，提示 rDNA 活性减弱。这种影响在情感障碍患者中更为明显，并且不能用抗抑郁药和抗精神病药来解释。
