Dhungana Sanjeev Kumar, Kim Il-Doo, Kwak Hwa-Sook, Shin Dong-Hyun
School of Applied Biosciences, Kyungpook National University, Daegu 702-701, Republic of Korea.
International Institute of Agricultural Research & Development, Kyungpook National University, Daegu 702-701, Republic of Korea.
Pestic Biochem Physiol. 2016 Jun;130:39-43. doi: 10.1016/j.pestbp.2015.12.002. Epub 2015 Dec 8.
Although a considerable number of studies about the effect of different insecticides on plant physiology and metabolism have been carried out, research work about the comparative action of structurally different classes of insecticide on physiological and biochemical properties of soybean seed germination and early growth has not been found. The objective of this study was to investigate the effect of different classes of insecticides on soybean seed germination and early plant growth. Soybean seeds of Bosuk cultivar were soaked for 24h in distilled water or recommended dose (2mLL(-1), 1mLL(-1), 0.5gL(-1), and 0.5gL(-1) water for insecticides Mepthion, Myungtaja, Actara, and Stonate, respectively) of pesticide solutions of four structurally different classes of insecticides - Mepthion (fenitrothion; organophosphate), Myungtaja (etofenprox; pyrethroid), Actara (thiamethoxam; neonicotinoid), and Stonate (lambda-cyhalothrin cum thiamethoxam; pyrethroid cum neonicotinoid) - which are used for controlling stink bugs in soybean crop. Insecticides containing thiamethoxam and lamda-cyhalothrin cum thiamethoxam showed positive effects on seedling biomass and content of polyphenol and flavonoid, however fenitrothion insecticide reduced the seed germination, seed and seedling vigor, and polyphenol and flavonoid contents in soybean. Results of this study reveal that different classes of insecticide have differential influence on physiologic and metabolic actions like germination, early growth, and antioxidant activities of soybean and this implies that yield and nutrient content also might be affected with the application of different types of insecticide.
尽管已经开展了大量关于不同杀虫剂对植物生理和代谢影响的研究，但尚未发现关于结构不同类别的杀虫剂对大豆种子萌发和早期生长的生理生化特性的比较作用的研究工作。本研究的目的是调查不同类别的杀虫剂对大豆种子萌发和早期植株生长的影响。将Bosuk品种的大豆种子在蒸馏水中或四种结构不同类别的杀虫剂——速灭磷（杀螟硫磷；有机磷酸酯类）、Myungtaja（醚菊酯；拟除虫菊酯类）、阿克泰（噻虫嗪；新烟碱类）和司通立（高效氯氟氰菊酯和噻虫嗪复配剂；拟除虫菊酯和新烟碱复配剂）——的农药溶液推荐剂量（分别为2mL L⁻¹、1mL L⁻¹、0.5g L⁻¹和0.5g L⁻¹水）中浸泡24小时，这些杀虫剂用于防治大豆作物中的椿象。含有噻虫嗪以及高效氯氟氰菊酯和噻虫嗪复配剂的杀虫剂对幼苗生物量以及多酚和类黄酮含量有积极影响，然而，杀螟硫磷杀虫剂降低了大豆的种子萌发、种子和幼苗活力以及多酚和类黄酮含量。本研究结果表明，不同类别的杀虫剂对大豆的萌发、早期生长和抗氧化活性等生理和代谢作用有不同影响，这意味着不同类型杀虫剂的施用也可能会影响产量和养分含量。
