Wu Si, Wu Yingying, Lu Yiping, Yue Yuanyi, Cui Changwan, Yu Miao, Wang Shuang, Liu Miao, Zhao Ying, Sun Zhengrong
Department of Biobank, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China.
Department of Gastroenterology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China.
Oncol Lett. 2020 Oct;20(4):28. doi: 10.3892/ol.2020.11889. Epub 2020 Jul 17.
Cervical cancer is the fourth leading cause of cancer-associated mortality worldwide. However, its underlying molecular mechanisms are unclear. It is important to explore these mechanisms in order to identify novel diagnostic and prognostic biomarkers. The present study determined the association between STAT1 and human papillomavirus (HPV)16 in cervical lesions. STAT1 expression was detected by immunohistochemistry. Quantitative PCR was used to detect HPV16 viral load and STAT1 expression in cervical lesions. The potential associations among STAT1 expression, HPV16 viral load and the severity of cervical lesions in patients were analyzed using receiver operating characteristic (ROC) curves. The Cancer Genome Atlas database was used to analyze STAT1 expression and survival. High STAT1 expression was observed in 10.71 (3/28), 41.18 (14/34), 53.06 (26/49) and 90.00% (27/30) of normal tissue, low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL) and cervical squamous cell carcinoma samples, respectively. The HPV16 copy number gradually increased with the progression of cervical lesions, with the highest copy number observed in cervical cancer samples. In addition, STAT1 expression was positively correlated with HPV16 viral load. Furthermore, ROC curve analysis demonstrated that the combination of STAT1 expression and HPV16 viral load was able to differentiate between LSIL/HSIL and cervical cancer samples. Bioinformatics analysis revealed that STAT1 expression was associated with improved survival in cervical cancer. Additionally, STAT1 expression was positively associated with the progression of cervical lesions, and HPV16 viral load may affect STAT1 expression. Overall, these findings indicate that STAT1 may be an indicator of the status of cervical lesions.
宫颈癌是全球癌症相关死亡的第四大主要原因。然而，其潜在的分子机制尚不清楚。探索这些机制对于识别新的诊断和预后生物标志物很重要。本研究确定了STAT1与宫颈病变中人类乳头瘤病毒（HPV）16之间的关联。通过免疫组织化学检测STAT1表达。采用定量PCR检测宫颈病变中HPV16病毒载量和STAT1表达。使用受试者工作特征（ROC）曲线分析患者中STAT1表达、HPV16病毒载量与宫颈病变严重程度之间的潜在关联。利用癌症基因组图谱数据库分析STAT1表达和生存率。在正常组织、低级别鳞状上皮内病变（LSIL）、高级别鳞状上皮内病变（HSIL）和宫颈鳞状细胞癌样本中，分别有10.71%（3/28）、41.18%（14/34）、53.06%（26/49）和90.00%（27/30）观察到STAT1高表达。随着宫颈病变进展，HPV16拷贝数逐渐增加，在宫颈癌样本中观察到最高拷贝数。此外，STAT1表达与HPV16病毒载量呈正相关。此外，ROC曲线分析表明，STAT1表达和HPV16病毒载量的组合能够区分LSIL/HSIL和宫颈癌样本。生物信息学分析显示，STAT1表达与宫颈癌患者生存率提高相关。此外，STAT1表达与宫颈病变进展呈正相关，HPV16病毒载量可能影响STAT1表达。总体而言，这些发现表明STAT1可能是宫颈病变状态的一个指标。
