Julien P, Despres J P, Angel A
Department of Medicine, University of Toronto, Ontario, Canada.
J Lipid Res. 1989 Feb;30(2):293-9.
To determine the effect of obesity on the size distribution of fat cell populations in human adipose tissue, omental fat tissue biopsies were obtained from lean, moderately obese, and massively obese patients. The size distributions of adipocytes from lean and obese fat tissues examined by the scanning electron microscopic method were bimodal, consisting of populations of very small fat cells and mature fat cells, in contrast to collagenase-derived isolated cells that showed only the large mature fat cells. The very small fat cell population represented 21 to 26% of the total fat cell number in the lean and in both obese groups. In contrast, preparations of human fat cells isolated by the collagenase method systematically excluded the very small fat cells. In massive obesity, both cell populations participated in the hyperplastic growth but only the larger mature fat cells increased in size, implying that these two cell populations differ in their physiological role.
为了确定肥胖对人体脂肪组织中脂肪细胞群体大小分布的影响，我们从体型偏瘦、中度肥胖和极度肥胖的患者身上获取了网膜脂肪组织活检样本。通过扫描电子显微镜法检测发现，偏瘦和肥胖脂肪组织中的脂肪细胞大小分布呈双峰模式，由非常小的脂肪细胞群体和成熟脂肪细胞群体组成，这与仅显示大型成熟脂肪细胞的胶原酶分离细胞形成对比。非常小的脂肪细胞群体在偏瘦组和两个肥胖组中占总脂肪细胞数量的21%至26%。相比之下，用胶原酶法分离的人体脂肪细胞制剂系统性地排除了非常小的脂肪细胞。在极度肥胖中，这两个细胞群体都参与了增生性生长，但只有较大的成熟脂肪细胞大小增加，这意味着这两个细胞群体在生理作用上有所不同。
