Bukkitgar Shikandar D, Shetti Nagaraj P
Department of Chemistry, K.L.E. Institute of Technology, Gokul, Hubli, affiliated to Visvesvaraya Technological University, 580030, Karnataka, India.
Department of Chemistry, K.L.E. Institute of Technology, Gokul, Hubli, affiliated to Visvesvaraya Technological University, 580030, Karnataka, India.
Mater Sci Eng C Mater Biol Appl. 2016 Aug 1;65:262-8. doi: 10.1016/j.msec.2016.04.045. Epub 2016 Apr 14.
A novel sensor for the determination of 5-fluorouracil was constructed by electrochemical deposition of methylene blue on surface of carbon paste electrode. The electrode surface morphology was studied using Atomic force microscopy and XRD. The electrochemical activity of modified electrode was characterized using cyclic voltammetry and differential pulse method. The developed sensor shows impressive enlargement in sensitivity of 5-fluorouracil determination. The peak currents obtained from differential pulse voltammetry was linear with concentration of 5-fluorouracil in the range 4×10(-5)-1×10(-7)M and detection limit and quantification limit were calculated to be 2.04nM and 6.18nM respectively. Further, the sensor was successfully applied in pharmaceutical and biological fluid sample analysis.
通过在碳糊电极表面电化学沉积亚甲基蓝构建了一种用于测定5-氟尿嘧啶的新型传感器。使用原子力显微镜和X射线衍射研究了电极表面形态。采用循环伏安法和差分脉冲法对修饰电极的电化学活性进行了表征。所开发的传感器在5-氟尿嘧啶测定灵敏度方面表现出显著提高。差分脉冲伏安法获得的峰电流与5-氟尿嘧啶浓度在4×10(-5)-1×10(-7)M范围内呈线性关系，检测限和定量限分别计算为2.04nM和6.18nM。此外，该传感器成功应用于药物和生物流体样品分析。
