Muthukumaran Magesh Kumar, Govindaraj Muthukumar, Raja Bharathi Kannan, J Arockia Selvi
Department of Chemistry, SRM Institute of Science and Technology Kattankulathur 603203 Tamil Nadu India
RSC Adv. 2023 Jan 19;13(5):2780-2794. doi: 10.1039/d2ra07147a. eCollection 2023 Jan 18.
It is difficult for the scientific community to develop a nonenzymatic sensing platform for extremely sensitive and selective detection of specific biomolecules, antibiotics, food adulterants, heavy metals, One of the most significant chemotherapy drugs, 5-fluorouracil (5-Fu), which is used to treat solid malignancies, has a fluorine atom in the fifth position of the uracil molecule. Recognizing the secure and effective dosing of drugs for chemotherapy continues to be a critical concern in cancer disease management. The maintenance of the optimal 5-Fu concentration is dependent on the presence of 5-Fu in biofluids. Herein we reported a conducting polymer encapsulated 2D material, PTh/h-BN for the efficient electrochemical detection of anticancer drug 5-Fu. Furthermore, the synthesized PTh/h-BN nanocomposite was confirmed by the High-Resolution Transmission Electron Microscope (HR-TEM), High-Resolution Scanning Electron Microscope (HR-SEM), X-ray diffraction (XRD), and Fourier-Transform Infrared Spectroscopy (FT-IR). The electrical resistance of PTh/h-BN modified GCE and its sensing performance towards 5-Fu were tested using Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV) studies respectively. The analytical performance of our proposed catalyst was tested using Differential Pulse Voltammetry (DPV), and the amperometry (- curve) method. From the results, our proposed PTh/h-BN nanocomposite-modified GCE shows enhanced sensing performance due to higher redox peak currents, large active surface area, and high electrical conductivity. Moreover, the nanohybrid shows enhanced sensing performances with quick response time, wide linear range, the lowest limit of detection, high sensitivity, and high selectivity in the presence of various interferents. Finally, the practical applicability of the proposed sensor was tested with real-world samples with very good recovery percentages.
对于科学界来说，开发一个用于极其灵敏且选择性地检测特定生物分子、抗生素、食品掺假物、重金属的非酶传感平台是困难的。一种最重要的化疗药物，用于治疗实体恶性肿瘤的5-氟尿嘧啶（5-Fu），在尿嘧啶分子的第五位有一个氟原子。在癌症疾病管理中，确定化疗药物安全有效的给药剂量仍然是一个关键问题。生物流体中5-Fu的存在对于维持最佳5-Fu浓度至关重要。在此，我们报道了一种导电聚合物封装的二维材料PTh/h-BN，用于高效电化学检测抗癌药物5-Fu。此外，通过高分辨率透射电子显微镜（HR-TEM）、高分辨率扫描电子显微镜（HR-SEM）、X射线衍射（XRD）和傅里叶变换红外光谱（FT-IR）对合成得到的PTh/h-BN纳米复合材料进行了表征。分别使用电化学阻抗谱（EIS）和循环伏安法（CV）研究测试了PTh/h-BN修饰玻碳电极（GCE）的电阻及其对5-Fu的传感性能。使用差分脉冲伏安法（DPV）和安培法（-曲线）对我们提出的催化剂的分析性能进行了测试。结果表明，我们提出的PTh/h-BN纳米复合材料修饰的GCE由于具有更高的氧化还原峰电流、大的活性表面积和高电导率，显示出增强的传感性能。此外，该纳米杂化物在存在各种干扰物的情况下，具有快速响应时间、宽线性范围、最低检测限、高灵敏度和高选择性，展现出增强的传感性能。最后，用实际样品测试了所提出传感器的实际适用性，回收率非常好。
