Hanson C A, Schnitzer B
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109-00602.
J Clin Lab Anal. 1989;3(1):2-7. doi: 10.1002/jcla.1860030103.
Cytologic evaluation of body fluids and fine needle aspirations (FNA) is frequently required in patients with hematologic diseases. In this study we have correlated immunophenotyping and DNA analysis by flow cytometry with cytologic findings and tissue biopsies from 20 patients with body fluid specimens and 5 with FNA. Nineteen of 25 cases, including all FNA cases, had an immunophenotype consistent with malignancy: 12 monoclonal B-cell lymphomas, 2 T-cell lymphomas, 2 T-ALL and 3 non-T-ALL. By cytologic examination, 14 of these 19 cases were positive for malignant cells, 2 suspicious and 3 negative; the latter 5 cases, including 2 FNA cases, had small monoclonal B-cell populations detected by flow cytometry. Six cases had a benign immunophenotype; cytologic examination was benign in 4 of these and suspicious for lymphoma in 2. Our results show the feasibility of using flow cytometry to evaluate body fluids or FNA and demonstrate that small malignant populations that may be missed by routine cytology can be detected by flow cytometry.
血液系统疾病患者常常需要对体液和细针穿刺抽吸物（FNA）进行细胞学评估。在本研究中，我们将流式细胞术的免疫表型分析和DNA分析与20例有体液标本的患者以及5例有FNA的患者的细胞学检查结果和组织活检进行了关联。25例病例中有19例，包括所有FNA病例，其免疫表型与恶性肿瘤一致：12例为单克隆B细胞淋巴瘤，2例为T细胞淋巴瘤，2例为T-ALL，3例为非T-ALL。通过细胞学检查，这19例病例中有14例恶性细胞呈阳性，2例可疑，3例阴性；后5例病例，包括2例FNA病例，通过流式细胞术检测到有小的单克隆B细胞群体。6例病例具有良性免疫表型；其中4例细胞学检查为良性，2例可疑为淋巴瘤。我们的结果表明了使用流式细胞术评估体液或FNA的可行性，并证明了流式细胞术能够检测到常规细胞学检查可能遗漏的小的恶性细胞群体。
