Key Laboratory for Advanced Materials & Institute of Fine Chemicals, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, P. R. China.
The Ministry-Province Jointly Constructed Base for State Key Lab-Shenzhen Key Laboratory of Chemical Biology, The Graduate School at Shenzhen, Tsinghua University , Shenzhen 518055, P. R. China.
ACS Appl Mater Interfaces. 2016 Jun 1;8(21):13601-6. doi: 10.1021/acsami.6b03223. Epub 2016 May 17.
This paper describes the simple construction of a unique class of supramolecular ensembles formed by electrostatic self-assembly between charged conjugated polymers and fluorophore-coupled glycoligands (glycoprobes) for the selective fluorogenic detection of receptor proteins at both the molecular and cellular levels. We show that positively and negatively charged diazobenzene-containing poly(p-phenylethynylenes) (PPEs) can be used to form stable fluorogenic probes with fluorescein-based (negatively charged) and rhodamine B based (positively charged) glycoprobes by electrostatic interaction. The structures of the ensembles have been characterized by spectroscopic and microscopic techniques. The supramolecular probes formed show quenched fluorescence in an aqueous buffer solution, which can be specifically recovered, in a concentration-dependent manner, through competitive complexation with a selective protein receptor, over a range of other unselective proteins. The ensembles also show selective fluorescence enhancement with a live cell that expresses the glycoligand receptor but not a control cell without receptor expression.
本文描述了一种独特的超分子聚集体的简单构建方法，该聚集体通过带电荷的共轭聚合物与荧光团偶联的糖配体（糖探针）之间的静电自组装形成，可用于在分子和细胞水平上选择性地检测受体蛋白。我们表明，带正电荷和负电荷的含二氮烯基的聚（对苯乙炔基）（PPE）可以通过静电相互作用与基于荧光素的（带负电荷）和罗丹明 B 基（带正电荷）糖探针形成稳定的荧光探针。通过光谱和显微镜技术对聚集体的结构进行了表征。形成的超分子探针在水性缓冲溶液中显示荧光猝灭，但通过与选择性蛋白质受体进行竞争性络合，可以以浓度依赖的方式特异性恢复，而其他非选择性蛋白质则不会。这些聚集体还表现出与表达糖配体受体的活细胞的选择性荧光增强，而与没有受体表达的对照细胞没有增强。
