Wang Chang-Zheng, He Xiao-Peng
Key Laboratory for Advanced Materials & Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai 200237, China.
Key Laboratory for Advanced Materials & Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai 200237, China.
Carbohydr Res. 2018 Jan 2;455:1-4. doi: 10.1016/j.carres.2017.10.021. Epub 2017 Nov 1.
We have developed a new series of glycoprobe-polymer dot ensembles for the fluorogenic, homogeneous detection of lectins. Electrostatic self-assembly between positively charged rhodamine-based glycosides and negatively charged poly(3-hexylthiophene-2,5-diyl)/poly(styrene-co-maleic anhydride) polymer dots produces the ensembles with a quenched fluorescence. Fluorescence spectroscopy showed that the ensembles exhibited a concentration-dependent fluorescence enhancement with selective lectins over a range of unselective lectins and proteins. This research provides insight into the development of simple fluorogenic probes for homogeneous lectin analyses based on the supramolecular assembly between polymeric nanoparticles and fluorescent glycoprobes.
我们开发了一系列用于荧光法、均相检测凝集素的新型糖探针-聚合物点组合体。带正电荷的罗丹明基糖苷与带负电荷的聚（3-己基噻吩-2,5-二亚基）/聚（苯乙烯-马来酸酐共聚物）聚合物点之间的静电自组装产生了具有猝灭荧光的组合体。荧光光谱表明，该组合体在一系列非选择性凝集素和蛋白质中，对选择性凝集素表现出浓度依赖性荧光增强。这项研究为基于聚合物纳米颗粒与荧光糖探针之间的超分子组装开发用于均相凝集素分析的简单荧光探针提供了思路。
