Zhao Chengjin, Shen Yifen, Tao Xuelei, Xu Jian, Lu Junjie, Liu Chao, Xu Zhiwei, Tang Qing, Tao Tao, Zhang Xiubing
Department of Neurosurgery, Nantong Second People Affiliated Hospital of Nantong University, 43 Xinglong Road, Nantong, 226001, Jiangsu Province, People's Republic of China.
Department of Immunology, Medical School of Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu Province, People's Republic of China.
J Mol Histol. 2016 Jun;47(3):297-304. doi: 10.1007/s10735-016-9678-z. Epub 2016 May 9.
Carboxyl-terminal binding protein 1 (CtBP1), up-regulated in various types of human cancers, has been functionally associated with proliferation, anti-apoptosis, and EMT in vitro studies. However, the functional significance of CtBP1 in the pathophysiology of glioma remains unknown. In the present study, we showed the expression of CtBP1 was markedly higher in glioma tissues compared with normal brain tissues by Western blot analysis. Immunohistochemical analysis revealed that CtBP1 mainly localized in the nucleus of glioma cells. Statistical analysis suggested the upregulation of CtBP1 was considerably correlated with the WHO grade (P < 0.05) and those patients with high CtBP1 levels exhibited shorter survival time (P < 0.01). Silencing CtBP1 by short hairpin RNAi caused an inhibition of cell migration. Moreover, knockdown of CtBP1 increases E-cadherin expression and decreases vimentin expression. These data uncovered that CtBP1 protein is a valuable marker of glioma pathogenic process and that CtBP1 can serve as a novel prognostic marker for glioma therapy.
羧基末端结合蛋白1（CtBP1）在多种人类癌症中上调，在体外研究中其功能与增殖、抗凋亡和上皮-间质转化相关。然而，CtBP1在胶质瘤病理生理学中的功能意义仍不清楚。在本研究中，我们通过蛋白质印迹分析表明，与正常脑组织相比，胶质瘤组织中CtBP1的表达明显更高。免疫组织化学分析显示，CtBP1主要定位于胶质瘤细胞的细胞核中。统计分析表明，CtBP1的上调与世界卫生组织（WHO）分级显著相关（P<0.05），且CtBP1水平高的患者生存时间较短（P<0.01）。通过短发夹RNA干扰沉默CtBP1会导致细胞迁移受到抑制。此外，敲低CtBP1会增加E-钙黏蛋白的表达并降低波形蛋白的表达。这些数据揭示，CtBP1蛋白是胶质瘤致病过程的一个有价值的标志物，并且CtBP1可作为胶质瘤治疗的一种新的预后标志物。
