Ates Ufuk, Gollu Gulnur, Kucuk Gonul, Billur Deniz, Bingol-Kologlu Meltem, Yılmaz Yavuz, Ozkan-Ulu Hulya, Bayram Pinar, Bagriacik Emin, Dindar Huseyin
Ankara University, School of Medicine, Department of Pediatric Surgery, Ankara, Turkey.
Ankara University School of Medicine, Department of Histology and Embryology, Ankara, Turkey.
Arch Argent Pediatr. 2016 Jun 1;114(3):243-7. doi: 10.5546/aap.2016.eng.243.
BACKGROUND/AIM: The aim of the present study was to find out if there is an increase in the expression of pro-apoptotic Bax and reduction in expression of anti-apoptotic Blc-2A1 in newborn intestines with necrotizing enterocolitis (NEC).
We compared 8 consecutive newborn patients undergoing bowel resection for NEC with 8 neonates undergoing intestinal resection for ileal atresia. Histopathological evaluation of tissue injury and apoptosis was performed by using light microscopic examination and TUNEL method. The mRNA level of apoptotic (CASP3, CASP6, CASP7, Bax, BIRC2) and anti-apoptotic genes were evaluated by PCR array method. Protein expression was assessed by immunohistochemistry.
We compared 8 consecutive newborn patients undergoing bowel resection for NEC with 8 neonates undergoing intestinal resection for ileal atresia. Histopathological evaluation of tissue injury and apoptosis was performed by using light microscopic examination and TUNEL method. The mRNA level of apoptotic (CASP3, CASP6, CASP7, Bax, BIRC2) and anti-apoptotic genes were evaluated by PCR array method. Protein expression was assessed by immunohistochemistry.
Our data in humannewborns suggest that alteration of the balance between pro-apoptotic Bax expression and anti-apoptotic Bcl-2A1 expression in the site of injury is a possible mechanism in the pathogenesis of NEC.
背景/目的：本研究旨在探究坏死性小肠结肠炎（NEC）新生儿的肠道中促凋亡蛋白Bax的表达是否增加以及抗凋亡蛋白Blc - 2A1的表达是否减少。
我们将8例因NEC接受肠切除的连续新生儿患者与8例因回肠闭锁接受肠切除的新生儿进行了比较。通过光学显微镜检查和TUNEL法对组织损伤和凋亡进行组织病理学评估。采用PCR阵列法评估凋亡相关基因（CASP3、CASP6、CASP7、Bax、BIRC2）和抗凋亡基因的mRNA水平。通过免疫组织化学评估蛋白表达。
我们将8例因NEC接受肠切除的连续新生儿患者与8例因回肠闭锁接受肠切除的新生儿进行了比较。通过光学显微镜检查和TUNEL法对组织损伤和凋亡进行组织病理学评估。采用PCR阵列法评估凋亡相关基因（CASP3、CASP6、CASP7、Bax、BIRC2）和抗凋亡基因的mRNA水平。通过免疫组织化学评估蛋白表达。
我们在人类新生儿中的数据表明，损伤部位促凋亡蛋白Bax表达与抗凋亡蛋白Bcl - 2A1表达之间的平衡改变可能是NEC发病机制中的一种机制。
