Townsend DeWayne
Department of Integrative Biology and Physiology, University of Minnesota;
J Vis Exp. 2016 May 2(111):53810. doi: 10.3791/53810.
Understanding the causes and progression of heart disease presents a significant challenge to the biomedical community. The genetic flexibility of the mouse provides great potential to explore cardiac function at the molecular level. The mouse's small size does present some challenges in regards to performing detailed cardiac phenotyping. Miniaturization and other advancements in technology have made many methods of cardiac assessment possible in the mouse. Of these, the simultaneous collection of pressure and volume data provides a detailed picture of cardiac function that is not available through any other modality. Here a detailed procedure for the collection of pressure-volume loop data is described. Included is a discussion of the principles underlying the measurements and the potential sources of error. Anesthetic management and surgical approaches are discussed in great detail as they are both critical to obtaining high quality hemodynamic measurements. The principles of hemodynamic protocol development and relevant aspects of data analysis are also addressed.
了解心脏病的病因和发展过程给生物医学界带来了重大挑战。小鼠的基因灵活性为在分子水平上探索心脏功能提供了巨大潜力。小鼠体型小在进行详细的心脏表型分析方面确实存在一些挑战。技术的小型化和其他进步使小鼠心脏评估的许多方法成为可能。其中，压力和容积数据的同步采集提供了心脏功能的详细情况，这是其他任何方式都无法提供的。本文描述了采集压力-容积环数据的详细程序。包括对测量基础原理和潜在误差来源的讨论。麻醉管理和手术方法进行了详细讨论，因为它们对于获得高质量的血流动力学测量都至关重要。还讨论了血流动力学方案制定的原则和数据分析的相关方面。
