• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

来自南非领土2型口蹄疫病毒的3C蛋白酶的晶体结构。

Crystal structure of the 3C protease from Southern African Territories type 2 foot-and-mouth disease virus.

作者信息

Yang Jingjie, Leen Eoin N, Maree Francois F, Curry Stephen

机构信息

Departmet of Life Sciences, Imperial College , London , United Kingdom.

Transboundary Animal Disease Programme, Agricultural Research Council, Onderstepoort Veterinary Institute , Onderstepoort , South Africa.

出版信息

PeerJ. 2016 Apr 26;4:e1964. doi: 10.7717/peerj.1964. eCollection 2016.

DOI:10.7717/peerj.1964
PMID:27168976
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4860321/
Abstract

The replication of foot-and-mouth disease virus (FMDV) is dependent on the virus-encoded 3C protease (3C(pro)). As in other picornaviruses, 3C(pro) performs most of the proteolytic processing of the polyprotein expressed from the large open reading frame in the RNA genome of the virus. Previous work revealed that the 3C(pro) from serotype A-one of the seven serotypes of FMDV-adopts a trypsin-like fold. On the basis of capsid sequence comparisons the FMDV serotypes are grouped into two phylogenetic clusters, with O, A, C, and Asia 1 in one, and the three Southern African Territories serotypes, (SAT-1, SAT-2 and SAT-3) in another, a grouping pattern that is broadly, but not rigidly, reflected in 3C(pro) amino acid sequences. We report here the cloning, expression and purification of 3C proteases from four SAT serotype viruses (SAT2/GHA/8/91, SAT1/NIG/5/81, SAT1/UGA/1/97, and SAT2/ZIM/7/83) and the crystal structure at 3.2 Å resolution of 3C(pro) from SAT2/GHA/8/91.

摘要

口蹄疫病毒（FMDV）的复制依赖于病毒编码的3C蛋白酶（3C(pro)）。与其他小RNA病毒一样，3C(pro)对病毒RNA基因组中大型开放阅读框所表达的多聚蛋白进行大部分的蛋白水解加工。先前的研究表明，FMDV七种血清型之一的A型3C(pro)具有胰蛋白酶样折叠结构。基于衣壳序列比较，FMDV血清型被分为两个系统发育簇，一个簇包含O、A、C和亚洲1型，另一个簇包含三个南非地区血清型（SAT-1、SAT-2和SAT-3），这种分组模式在3C(pro)氨基酸序列中大致但并非严格地有所体现。我们在此报告了四种SAT血清型病毒（SAT2/GHA/8/91、SAT1/NIG/5/81、SAT1/UGA/1/97和SAT2/ZIM/7/83）的3C蛋白酶的克隆、表达和纯化，以及SAT2/GHA/8/91的3C(pro)在3.2 Å分辨率下的晶体结构。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97c9/4860321/b3804ddb5296/peerj-04-1964-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97c9/4860321/0f59d893203c/peerj-04-1964-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97c9/4860321/b3804ddb5296/peerj-04-1964-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97c9/4860321/0f59d893203c/peerj-04-1964-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/97c9/4860321/b3804ddb5296/peerj-04-1964-g002.jpg

相似文献

1
Crystal structure of the 3C protease from Southern African Territories type 2 foot-and-mouth disease virus.来自南非领土2型口蹄疫病毒的3C蛋白酶的晶体结构。
PeerJ. 2016 Apr 26;4:e1964. doi: 10.7717/peerj.1964. eCollection 2016.
2
Determination of common genetic variants within the non-structural proteins of foot-and-mouth disease viruses isolated in sub-Saharan Africa.
Vet Microbiol. 2015 May 15;177(1-2):106-22. doi: 10.1016/j.vetmic.2015.03.007. Epub 2015 Mar 14.
3
Genetic heterogeneity in the foot-and-mouth disease virus Leader and 3C proteinases.口蹄疫病毒前导蛋白酶和3C蛋白酶中的遗传异质性。
Gene. 2002 May 1;289(1-2):19-29. doi: 10.1016/s0378-1119(02)00471-7.
4
Development of reverse-transcriptase, real-time PCR assays to distinguish the Southern African Territories (SAT) serotypes 1 and 3 and topotype VII of SAT2 of Foot-and-Mouth Disease Virus.用于区分口蹄疫病毒南非领土（SAT）血清型1和3以及SAT2的VII型拓扑型的逆转录酶实时PCR检测方法的开发。
Front Vet Sci. 2022 Sep 20;9:977761. doi: 10.3389/fvets.2022.977761. eCollection 2022.
5
Viral Population Diversity during Co-Infection of Foot-And-Mouth Disease Virus Serotypes SAT1 and SAT2 in African Buffalo in Kenya.肯尼亚非洲水牛中口蹄疫病毒血清型 SAT1 和 SAT2 共感染期间的病毒群体多样性。
Viruses. 2022 Apr 25;14(5):897. doi: 10.3390/v14050897.
6
Inherent biophysical stability of foot-and-mouth disease SAT1, SAT2 and SAT3 viruses.口蹄疫 SAT1、SAT2 和 SAT3 病毒的固有生物物理稳定性。
Virus Res. 2019 Apr 15;264:45-55. doi: 10.1016/j.virusres.2019.02.012. Epub 2019 Feb 23.
7
Development of a New RT-PCR with Multiple Primers for Detecting Southern African Territories Foot-and-mouth Disease Viruses.用于检测南部非洲地区口蹄疫病毒的新型多重引物逆转录聚合酶链反应的开发
J Vet Res. 2018 Dec 31;62(4):431-437. doi: 10.2478/jvetres-2018-0064. eCollection 2018 Dec.
8
Crystal structure of foot-and-mouth disease virus 3C protease. New insights into catalytic mechanism and cleavage specificity.口蹄疫病毒3C蛋白酶的晶体结构。对催化机制和切割特异性的新见解。
J Biol Chem. 2005 Mar 25;280(12):11520-7. doi: 10.1074/jbc.M413254200. Epub 2005 Jan 14.
9
Predicting antigenic sites on the foot-and-mouth disease virus capsid of the South African Territories types using virus neutralization data.利用病毒中和数据预测南非领土型口蹄疫病毒衣壳上的抗原位点。
J Gen Virol. 2011 Oct;92(Pt 10):2297-2309. doi: 10.1099/vir.0.032839-0. Epub 2011 Jun 22.
10
Complex Circulation of Foot-and-Mouth Disease Virus in Cattle in Nigeria.尼日利亚牛群中口蹄疫病毒的复杂传播情况
Front Vet Sci. 2020 Aug 20;7:466. doi: 10.3389/fvets.2020.00466. eCollection 2020.

引用本文的文献

1
Structural Biology of the Enterovirus Replication-Linked 5'-Cloverleaf RNA and Associated Virus Proteins.肠道病毒复制相关 5'-叶状环 RNA 及其相关病毒蛋白的结构生物学。
Microbiol Mol Biol Rev. 2020 Mar 18;84(2). doi: 10.1128/MMBR.00062-19. Print 2020 May 20.
2
Expression, purification, crystallization and preliminary X-ray diffraction analysis of swine leukocyte antigen 2 complexed with a CTL epitope AS64 derived from Asia1 serotype of foot-and-mouth disease virus.与源自口蹄疫病毒Asia1血清型的CTL表位AS64复合的猪白细胞抗原2的表达、纯化、结晶及初步X射线衍射分析
BMC Vet Res. 2018 Dec 18;14(1):407. doi: 10.1186/s12917-018-1742-3.
3

本文引用的文献

1
Predicting antigenic sites on the foot-and-mouth disease virus capsid of the South African Territories types using virus neutralization data.利用病毒中和数据预测南非领土型口蹄疫病毒衣壳上的抗原位点。
J Gen Virol. 2011 Oct;92(Pt 10):2297-2309. doi: 10.1099/vir.0.032839-0. Epub 2011 Jun 22.
2
Features and development of Coot.Coot的特点与发展
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501. doi: 10.1107/S0907444910007493. Epub 2010 Mar 24.
3
PHENIX: a comprehensive Python-based system for macromolecular structure solution.
On the helical arrangements of protein molecules.
蛋白质分子的螺旋排列。
Protein Sci. 2018 Mar;27(3):643-652. doi: 10.1002/pro.3356. Epub 2017 Dec 20.
PHENIX：一个基于Python的用于大分子结构解析的综合系统。
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21. doi: 10.1107/S0907444909052925. Epub 2010 Jan 22.
4
Insights into cleavage specificity from the crystal structure of foot-and-mouth disease virus 3C protease complexed with a peptide substrate.从与肽底物结合的口蹄疫病毒 3C 蛋白酶复合物的晶体结构中获得的断裂特异性见解。
J Mol Biol. 2010 Jan 15;395(2):375-89. doi: 10.1016/j.jmb.2009.10.048. Epub 2009 Oct 31.
5
Phaser crystallographic software.相位结晶学软件。
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674. doi: 10.1107/S0021889807021206. Epub 2007 Jul 13.
6
Toward rational protein crystallization: A Web server for the design of crystallizable protein variants.迈向理性蛋白质结晶：用于设计可结晶蛋白质变体的网络服务器。
Protein Sci. 2007 Aug;16(8):1569-76. doi: 10.1110/ps.072914007.
7
Structural analysis of foot-and-mouth disease virus 3C protease: a viable target for antiviral drugs?口蹄疫病毒3C蛋白酶的结构分析：抗病毒药物的可行靶点？
Biochem Soc Trans. 2007 Jun;35(Pt 3):594-8. doi: 10.1042/BST0350594.
8
Structural and mutagenic analysis of foot-and-mouth disease virus 3C protease reveals the role of the beta-ribbon in proteolysis.口蹄疫病毒3C蛋白酶的结构与诱变分析揭示了β-带在蛋白水解中的作用。
J Virol. 2007 Jan;81(1):115-24. doi: 10.1128/JVI.01587-06. Epub 2006 Oct 25.
9
Foot-and-mouth disease virus 3C protease: recent structural and functional insights into an antiviral target.口蹄疫病毒3C蛋白酶：抗病毒靶点的最新结构与功能见解
Int J Biochem Cell Biol. 2007;39(1):1-6. doi: 10.1016/j.biocel.2006.07.006. Epub 2006 Aug 14.
10
Dual modes of modification of hepatitis A virus 3C protease by a serine-derived beta-lactone: selective crystallization and formation of a functional catalytic triad in the active site.丝氨酸衍生的β-内酰胺对甲型肝炎病毒3C蛋白酶的双重修饰模式：活性位点中选择性结晶及功能性催化三联体的形成
J Mol Biol. 2005 Dec 9;354(4):854-71. doi: 10.1016/j.jmb.2005.09.074. Epub 2005 Oct 14.