Laboratory of Analytical Chemistry, Department of Chemistry, University of Crete, Heraklion, 70013 Crete, Greece.
Laboratory of Biochemistry, Department of Chemistry, University of Crete, Heraklion, 70013 Crete, Greece.
Analyst. 2016 Jun 20;141(13):4170-80. doi: 10.1039/c6an00783j.
In this work we report on the optical properties of specific synthetic carbon nano-dots (CDs) and their suitability for the development of optical biosensors. We examine the photoluminescence behavior of these CDs under different conditions, in their native form, as well as when conjugated to the catalytic protein glucose oxidase (GOx) for the construction of optical glucose biosensors. The effect of pH and hydrogen peroxide on the observed spectra is examined as the basis for the biosensor development. The CDs examined here have inherent surface amino functional groups which allow for easy conjugation to biomolecules via EDC-NHS, providing a well defined platform for biosensing applications. We conclude that the well controlled, stable, and highly efficient fluorescence behavior of the CDs in solution or in conjugate, provides the grounds for this class of materials to be used in a variety of arrangements for the development of optical and optoelectrochemical detection systems.
在这项工作中，我们报告了特定合成碳纳米点（CDs）的光学性质及其在开发光学生物传感器方面的适用性。我们研究了这些 CDs 在不同条件下的光致发光行为，包括它们的天然形式以及与催化蛋白葡萄糖氧化酶（GOx）共轭时用于构建光学葡萄糖生物传感器的形式。我们考察了 pH 值和过氧化氢对观察到的光谱的影响，以此作为生物传感器开发的基础。这里研究的 CDs 具有固有的表面氨基官能团，可通过 EDC-NHS 轻松与生物分子偶联，为生物传感应用提供了一个明确的平台。我们得出结论，CDs 在溶液或共轭物中具有良好控制、稳定和高效的荧光行为，为这类材料在各种用于开发光学和光电化学检测系统的配置中得到应用提供了依据。
