Wang Z J, Wang H X, Li L, Wang L, Dou H H
Department of Emergency Surgery, The First Affiliated Hospital of Bengbu medical College, Bengbu, Anhui, China.
Jiaozuo Coal Industry (Group) Co., Ltd. Central Hospital Department of orthopedics, Jiaozuo, Henan, China.
Genet Mol Res. 2016 Apr 28;15(2):gmr8181. doi: 10.4238/gmr.15028181.
We investigated the influence of different fluid resuscitation techniques on the number of myeloid-derived suppressor cells (MDSCs) in rats. Seventy-two healthy Sprague-Dawley rats were randomly divided into groups that received sham operation (Sham group), hypertonic saline (HRS group), lactated ringer's solution (LRS group), or crystalloid solution (LCRS group). Six rats from each group were sacrificed by cervical dislocation at 12, 24, and 48 h after resuscitation. The spleens were harvested under sterile conditions and spleen cell suspension was prepared. The number of MDSCs was detected using flow cytometry. The number of MDSCs in the Sham group did not differ significantly among the different time points. Compared with the Sham group, the number of MDSCs after the use of the different fluid resuscitation techniques increased to varying extents and the differences among the groups were significant. The number of MDSCs in the HRS group was much lower than that of the LRS and LCRS groups at both 24 and 48 h (P < 0.05). At 12 h, the number of MDSCs in the HRS group was significantly lower than that of the LRS group (P < 0.05). The differences between the HRS and LCRS groups were not statistically significant. Shortly after hemorrhagic shock resuscitation, the immune function of rats was suppressed to a varying extent and was gradually restored over time. Resuscitation with HRS alleviated the immunosuppression at the early stage after shock.
我们研究了不同液体复苏技术对大鼠骨髓来源抑制细胞（MDSCs）数量的影响。将72只健康的Sprague-Dawley大鼠随机分为接受假手术的组（假手术组）、高渗盐水组（HRS组）、乳酸林格液组（LRS组）或晶体液组（LCRS组）。复苏后12、24和48小时，每组处死6只大鼠，通过颈椎脱臼法进行。在无菌条件下采集脾脏并制备脾细胞悬液。使用流式细胞术检测MDSCs的数量。假手术组不同时间点的MDSCs数量差异无统计学意义。与假手术组相比，使用不同液体复苏技术后MDSCs数量均有不同程度增加，且组间差异有统计学意义。在24和48小时时，HRS组的MDSCs数量均显著低于LRS组和LCRS组（P<0.05）。在12小时时，HRS组的MDSCs数量显著低于LRS组（P<0.05）。HRS组和LCRS组之间的差异无统计学意义。失血性休克复苏后不久，大鼠的免疫功能受到不同程度抑制，并随时间逐渐恢复。使用HRS进行复苏可减轻休克后早期的免疫抑制。
