Jiang Jiu-Kun, Fang Wen, Hong Liang-Jie, Lu Yuan-Qiang
Department of Emergency Medicine, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.
Key Laboratory of Combined Multi-organ Transplantation, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.
J Zhejiang Univ Sci B. 2017;18(1):48-58. doi: 10.1631/jzus.B1600510.
To investigate the distribution and differentiation of myeloid-derived suppressor cells (MDSCs) in hemorrhagic shock mice, which are resuscitated with normal saline (NS), hypertonic saline (HTS), and hydroxyethyl starch (HES).
BALB/c mice were randomly divided into control, NS, HTS, and HES resuscitation groups. Three subgroups (n=8) in each resuscitation group were marked as 2, 24, and 72 h. Flow cytometry was used to detect the MDSCs, monocytic MDSCs (M-MDSCs), and granulocytic/neutrophilic MDSCs (G-MDSCs) in peripheral blood nucleated cells (PBNCs), spleen single-cell suspension, and bone marrow nucleated cells (BMNCs).
The MDSCs in BMNCs among three resuscitation groups were lower 2 h after shock, in PBNCs of the HTS group were higher, and in spleen of the NS group were lower (all P<0.05 vs. control). The M-MDSC/G-MDSC ratios in PBNCs of the HTS and HES groups were lower (both P<0.05 vs. control). At 24 h, the MDSCs in PBNCs of the NS and HTS groups were higher, while the spleen MDSCs in the HTS group were higher (all P<0.05 vs. control). The M-MDSC/G-MDSC ratios were all less in PBNCs, spleen, and BMNCs of the NS and HTS groups, and were lower in BMNCs of the HES group (all P<0.05 vs. control). At 72 h, the elevated MDSCs in PBNCs were presented in the HTS and HES groups, and in spleen the augment turned up in three resuscitation groups (all P<0.05 vs. control). The inclined ratios to M-MDSC were exhibited in spleen of the NS and HTS groups, and in PBNCs of the NS group; the inclination to G-MDSC in BMNCs was shown in the HES group (all P<0.05 vs. control).
HTS induces the earlier elevation of MDSCs in peripheral blood and spleen, and influences its distribution and differentiation, while HES has a less effect on the distribution but a stronger impact on the differentiation of MDSCs, especially in bone marrow.
研究用生理盐水（NS）、高渗盐水（HTS）和羟乙基淀粉（HES）复苏的失血性休克小鼠骨髓来源抑制细胞（MDSCs）的分布及分化情况。
将BALB/c小鼠随机分为对照组、NS复苏组、HTS复苏组和HES复苏组。每个复苏组再分为3个亚组（n = 8），分别标记为2小时、24小时和72小时。采用流式细胞术检测外周血有核细胞（PBNCs）、脾脏单细胞悬液和骨髓有核细胞（BMNCs）中的MDSCs、单核细胞来源的MDSCs（M-MDSCs）和粒细胞/中性粒细胞来源的MDSCs（G-MDSCs）。
休克后2小时，三个复苏组BMNCs中的MDSCs均降低，HTS组PBNCs中的MDSCs升高，NS组脾脏中的MDSCs降低（与对照组相比，均P<0.05）。HTS组和HES组PBNCs中的M-MDSC/G-MDSC比值降低（与对照组相比，均P<0.05）。24小时时，NS组和HTS组PBNCs中的MDSCs升高，HTS组脾脏中的MDSCs升高（与对照组相比，均P<0.05）。NS组和HTS组PBNCs、脾脏和BMNCs中的M-MDSC/G-MDSC比值均降低，HES组BMNCs中的该比值降低（与对照组相比，均P<0.05）。72小时时，HTS组和HES组PBNCs中的MDSCs升高，三个复苏组脾脏中的MDSCs均升高（与对照组相比，均P<0.05）。NS组和HTS组脾脏中以及NS组PBNCs中M-MDSC比值呈上升趋势；HES组BMNCs中G-MDSC比值呈上升趋势（与对照组相比，均P<0.05）。
HTS可使外周血和脾脏中的MDSCs较早升高，并影响其分布和分化，而HES对MDSCs分布影响较小，但对其分化影响较大，尤其是在骨髓中。
