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GTm6AC序列过度缠绕且弯曲。

The GTm6AC sequence is overwound and bent.

作者信息

Fazakerley G V, Gabarro-Arpa J, Lebret M, Guy A, Guschlbauer W

机构信息

Département de Biologie, Centre d'Etudes Nucléaires de Saclay, Gif-sur-Yvette, France.

出版信息

Nucleic Acids Res. 1989 Apr 11;17(7):2541-56. doi: 10.1093/nar/17.7.2541.

DOI:10.1093/nar/17.7.2541
PMID:2717401
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC317642/
Abstract

By a combination of distance constraints obtained from NMR spectra and molecular mechanics calculations we have determined the three dimensional structure of the self-complementary decanucleotide d(CGCGTm6ACGCG). Methylation of an adenine at a position 3' to T induces significant conformational changes relative to B-DNA. This arises from the close proximity of the four methyl groups in the large groove in the centre of the sequence. The helical twist between the two T.m6A base pairs is found to be 45 degrees, as for D-DNA, and is accompanied by a high negative value of the wedge roll angle between these base pairs. The overall nonzero wedge roll observed shows that the helix is bent. These constraints appear to be material for the absence of the sequence T-m6A in natural DNAs.

摘要

通过结合从核磁共振光谱获得的距离限制和分子力学计算，我们确定了自互补十聚体d(CGCGTm6ACGCG)的三维结构。相对于B - DNA，在T的3'位置的腺嘌呤甲基化会引起显著的构象变化。这是由于序列中心大沟中四个甲基的紧密接近所致。发现两个T.m6A碱基对之间的螺旋扭转角度为45度，与D - DNA相同，并且这些碱基对之间的楔形滚动角具有很高的负值。观察到的整体非零楔形滚动表明螺旋是弯曲的。这些限制似乎是天然DNA中不存在T - m6A序列的原因。

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Effects of methylation on the stability of nucleic acid conformations: studies at the monomer level.甲基化对核酸构象稳定性的影响：单体水平的研究
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An NMR study of A-T base pair opening rates in oligonucleotides. Influence of sequence and of adenine methylation.寡核苷酸中A-T碱基对打开速率的核磁共振研究。序列和腺嘌呤甲基化的影响。
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