Zong Wei, Hu Ying, Su Yingchun, Luo Nan, Zhang Xunan, Li Qingchuan, Han Xiaojun
a State Key Laboratory of Urban Water Resource and Environment, School of Chemical Engineering and Technology , Harbin Institute of Technology , Harbin , China ;
b School of Life Science and Technology , Harbin Institute of Technology , Harbin , China.
J Microencapsul. 2016 May;33(3):257-62. doi: 10.3109/02652048.2016.1156176. Epub 2016 May 12.
Stimuli-responsive drug carriers are considered to play important roles in chemotherapy. We fabricated pH-sensitive polydopamine-protected liposomes (liposome@PDA) drug delivery systems, which were characterised with microscope, scanning electron microscope (SEM), UV-vis spectrometer and Fourier transform infrared (FTIR) technieques. The typical chemotherapeutic agent, 5-fluorouracil (5-FU), was loaded into liposome@PDA capsules. The maximum release percentages of 5-FU are 3.2%, 29.5%, 52.7%, 76.7% in the solution with pH 7.42, 6.87, 4.11 and 3.16, respectively. The in vitro cell cytotoxity experiments were carried out using 5-FU-loaded capsules at pH 6.87 solution, which simulate the true pH around cancerous cells. At 1.5 μM concentration, the free 5-FU, 5-FU-loaded liposome capsules and 5-FU-loaded capsules showed the cell viability of 50.56%, 22.66% and 21.63%, respectively. It confirms that drug-loaded capsules performed better than free drug. The results demonstrate the great potential of liposome@PDA capsules as carriers in biomedical applications.
刺激响应型药物载体被认为在化疗中发挥着重要作用。我们制备了pH敏感的聚多巴胺保护脂质体（脂质体@PDA）药物递送系统，并用显微镜、扫描电子显微镜（SEM）、紫外可见光谱仪和傅里叶变换红外（FTIR）技术对其进行了表征。将典型的化疗药物5-氟尿嘧啶（5-FU）载入脂质体@PDA胶囊中。在pH值分别为7.42、6.87、4.11和3.16的溶液中，5-FU的最大释放百分比分别为3.2%、29.5%、52.7%、76.7%。使用载有5-FU的胶囊在pH 6.87溶液中进行体外细胞细胞毒性实验，该溶液模拟癌细胞周围的真实pH值。在1.5μM浓度下，游离5-FU、载有5-FU的脂质体胶囊和载有5-FU的胶囊的细胞活力分别为50.56%、22.66%和21.63%。这证实了载药胶囊的性能优于游离药物。结果表明脂质体@PDA胶囊作为生物医学应用中的载体具有巨大潜力。
