Mu Pengqian, Xu Nana, Chai Tingting, Jia Qi, Yin Zhiqiang, Yang Shuming, Qian Yongzhong, Qiu Jing
Institute of Quality Standards & Testing Technology for Agro-Products, Key Laboratory of Risk Monitoring and Assessment, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.
Institute of Quality Standards & Testing Technology for Agro-Products, Key Laboratory of Risk Monitoring and Assessment, Chinese Academy of Agricultural Sciences; Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing 100081, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Jun 15;1023-1024:17-23. doi: 10.1016/j.jchromb.2016.04.036. Epub 2016 Apr 30.
A fast method for the simultaneous determination of 14 antiviral drugs and relevant metabolites in chicken muscle by ultra-high liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed. The analytes included anti-influenza drugs (amantadine, rimantadine, oseltamivir, oseltamivir carboxylate, memantine, arbidol, and moroxydine), anti-herpes drugs (acyclovir, ganciclovir, famciclovir, penciclovir, ribavirin and its main metabolite TCONH2), and an immunomodulator (imiquimod). The samples were pretreated using a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method. The determination of the target compounds was conducted in less than 11.0min, and specificity was ensured by the use of multiple reaction monitoring (MRM) acquisition mode. Good linearities (R(2)>0.9928) were obtained in the range of 0.1-100μg/L, and the recovery rates were 56.2-113.4% with RSDs of 1.7-10.3% for intra-day precision and 2.4-8.8% for inter-day precision. The LODs and LOQs of all analytes were in the ranges of 0.02-1.0 and 0.05-2.5μg/kg, respectively. An analysis of real samples suggested that this method is simple, sensitive, reliable and practical for the detection of antiviral drugs in animal tissue.
建立了一种超高效液相色谱串联质谱（UPLC-MS/MS）同时测定鸡肉中14种抗病毒药物及其相关代谢物的快速方法。分析物包括抗流感药物（金刚烷胺、金刚乙胺、奥司他韦、奥司他韦羧酸盐、美金刚、阿比多尔、吗啉胍）、抗疱疹药物（阿昔洛韦、更昔洛韦、泛昔洛韦、喷昔洛韦、利巴韦林及其主要代谢物TCONH2）和一种免疫调节剂（咪喹莫特）。样品采用改良的QuEChERS（快速、简便、廉价、有效、耐用和安全）方法进行预处理。在不到11.0分钟内完成目标化合物的测定，并通过多反应监测（MRM）采集模式确保特异性。在0.1-100μg/L范围内获得良好的线性关系（R(2)>0.9928），日内精密度的回收率为56.2-113.4%，相对标准偏差为1.7-10.3%，日间精密度的相对标准偏差为2.4-8.8%。所有分析物的检测限和定量限分别在0.02-1.0和0.05-2.5μg/kg范围内。实际样品分析表明，该方法对于动物组织中抗病毒药物的检测简单、灵敏、可靠且实用。
