Center for Nonlinear Phenomena and Complex Systems, Université Libre de Bruxelles, Code Postal 231, Campus Plaine, B-1050 Brussels, Belgium.
Phys Rev E. 2016 Apr;93:042420. doi: 10.1103/PhysRevE.93.042420. Epub 2016 Apr 29.
Kinetic theory and thermodynamics are applied to DNA polymerases with exonuclease activity, taking into account the dependence of the rates on the previously incorporated nucleotide. The replication fidelity is shown to increase significantly thanks to this dependence at the basis of the mechanism of exonuclease proofreading. In particular, this dependence can provide up to a 100-fold lowering of the error probability under physiological conditions. Theory is compared with numerical simulations for the DNA polymerases of T7 viruses and human mitochondria.
将动力学理论和热力学应用于具有核酸外切酶活性的 DNA 聚合酶，同时考虑到速率对先前掺入核苷酸的依赖性。证明这种依赖关系是外切核酸酶校对机制的基础，可以显著提高复制保真度。特别是，这种依赖性可以在生理条件下将错误概率降低 100 倍。理论与 T7 病毒和人类线粒体的 DNA 聚合酶的数值模拟进行了比较。
