Nagai H, Yakuo I, Aoki M, Teshima K, Ono Y, Sengoku T, Shimazawa T, Aburada M, Koda A
Planta Med. 1989 Feb;55(1):13-7. doi: 10.1055/s-2006-961766.
The hepatoprotective effect of Gomisin A (TJN-101), which is a lignan compound isolated from Schizandra fruits, was studied on three immunologic liver injury models in mice. The first liver injury model was produced by the injection of anti-basic liver protein (BLP) antibody into DBA/2 mice which had been previously immunized with rabbit IgG (RGG). Other models were effected by injection of anti-liver specific protein (LSP) antibody into DBA/2 mice or by the injection of bacterial lipopolysaccharide (LPS) into ddY mice pretreated with Corynebacterium parvum (C. parvum). TJN-101 inhibited the elevation of transaminase (GOT and GPT) activities and showed the tendency to inhibit the histopathological changes of the liver in all models. Moreover, TJN-101 inhibited deoxycholic acid-induced release of transaminase from cultured rat hepatocytes in vitro, but did not affect the formation of hemolytic plaque forming cells in immunized mice spleens and hemolytic activity of guinea pig complement in immunohemolysis reaction. These results, therefore, suggested that the hepatoprotective effect of TJN-101 could be related to the protecting effect of hepatocyte plasma membrane rather than the inhibiting effects of the antibody formation and complement activity.
从五味子果实中分离得到的木脂素化合物戈米辛A（TJN - 101）对小鼠的三种免疫性肝损伤模型的保肝作用进行了研究。第一种肝损伤模型是通过向预先用兔免疫球蛋白（RGG）免疫的DBA/2小鼠注射抗碱性肝蛋白（BLP）抗体产生的。其他模型是通过向DBA/2小鼠注射抗肝特异性蛋白（LSP）抗体或向用短小棒状杆菌（C. parvum）预处理的ddY小鼠注射细菌脂多糖（LPS）来实现的。在所有模型中，TJN - 101均抑制了转氨酶（GOT和GPT）活性的升高，并显示出抑制肝脏组织病理学变化的趋势。此外，TJN - 101在体外抑制了脱氧胆酸诱导的培养大鼠肝细胞中转氨酶的释放，但不影响免疫小鼠脾脏中溶血空斑形成细胞的形成以及免疫溶血反应中豚鼠补体的溶血活性。因此，这些结果表明，TJN - 101的保肝作用可能与对肝细胞质膜的保护作用有关，而不是与抗体形成和补体活性的抑制作用有关。
