Mizoguchi Y, Shin T, Kobayashi K, Morisawa S
Third Department of Internal Medicine, Osaka City University Medical School, Japan.
Planta Med. 1991 Feb;57(1):11-4. doi: 10.1055/s-2006-960006.
Guinea pigs were sensitized with trinitrophenylated liver macromolecular protein fraction (TNP-LP1) prepared by using sodium trinitrobenzenesulfonate of strong immunogenicity as the hapten and LP1 as the carrier protein. The administration of trinitrophenylated hepatocytes and lipopolysaccharide to these TNP-LP1-sensitized guinea pigs through the mesenteric vein 2 weeks later resulted in the induction of acute hepatic failure accompanied by massive hepatic cell necrosis in almost all of the guinea pigs. Using this experimental model, the effect of Gomisin A on the induction of immunological acute hepatic failure was examined. As a result, the administration of gomisin A remarkably improved the survival rate and serum transaminase levels of the immunologically-induced acute hepatic failure guinea pigs. Gomisin A also improved the histological changes of the liver in these guinea pigs. These results suggested that gomisin A is effective for the improvement of immunologically-induced acute hepatic failure in our experimental model.
用具有强免疫原性的三硝基苯磺酸钠作为半抗原、LP1作为载体蛋白制备的三硝基苯基化肝大分子蛋白组分（TNP-LP1）对豚鼠进行致敏。2周后，通过肠系膜静脉向这些TNP-LP1致敏的豚鼠注射三硝基苯基化肝细胞和脂多糖，几乎所有豚鼠均诱发急性肝衰竭，并伴有大量肝细胞坏死。利用该实验模型，研究了五味子素A对免疫性急性肝衰竭诱导的影响。结果，给予五味子素A显著提高了免疫诱导的急性肝衰竭豚鼠的存活率和血清转氨酶水平。五味子素A还改善了这些豚鼠肝脏的组织学变化。这些结果表明，在我们的实验模型中，五味子素A对改善免疫诱导的急性肝衰竭有效。
