Wu Cong, Xu Zheng, Huang Kehe
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China.
Department of Biostatistics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Biol Trace Elem Res. 2016 Dec;174(2):428-435. doi: 10.1007/s12011-016-0735-y. Epub 2016 May 13.
Selenium (Se) is an essential trace element for humans and animals and is associated with many physiological functions. Previous studies have shown that low-Se diet may affect inflammatory cytokine productions and histology in the digestive system and that sulfide hydrogen (HS) may contribute to the protection against tissue injury and the inhibition of inflammation in the gastrointestinal tract. In this study, we investigated the relationship between Se deficiency-induced inflammation and HS production in the small intestine in chickens. One hundred twenty 1-day-old chickens were fed with diets with different Se concentrations (0.15 mg/kg in the control and 0.028 mg/kg in the low-Se-diet group). Chickens were euthanized and small intestinal tissues were extracted. We observed histology, measured HS concentration, and evaluated the mRNA expression of HS-producing enzymes cystathionine γ-lyase (CSE), cystathionine β-synthase (CBS), and 3-mercaptopyruvate sulfurtransferase (3-MST), and inflammatory factors TNF-α, NF-κB p50, COX-2, and PTGES. Our results showed that chickens fed with low-Se diet exhibited histological changes, lower HS production, and lower mRNA expression of HS-producing enzymes (CSE, CBS, and 3-MST) whereas higher mRNA expression of intestinal inflammatory factors (TNF-α, NF-κB p50, COX-2, and PTGES) compared to controls. Our results indicate that low-Se diet could impact HS, HS-producing enzymes, and inflammatory factors in the small intestine, implying that Se is important in maintaining intestinal functions and HS production is downregulated in Se deficiency-induced inflammation. The downregulation exacerbates the inflammation and impacts HS-mediated intestinal functions.
硒（Se）是人和动物必需的微量元素，与多种生理功能相关。先前的研究表明，低硒饮食可能会影响消化系统中炎性细胞因子的产生和组织学，并且硫化氢（HS）可能有助于预防组织损伤和抑制胃肠道炎症。在本研究中，我们调查了鸡小肠中硒缺乏诱导的炎症与HS产生之间的关系。120只1日龄鸡被喂食不同硒浓度的日粮（对照组为0.15mg/kg，低硒日粮组为0.028mg/kg）。对鸡实施安乐死后，提取小肠组织。我们观察了组织学，测量了HS浓度，并评估了产生HS的酶胱硫醚γ-裂解酶（CSE）、胱硫醚β-合酶（CBS）和3-巯基丙酮酸硫转移酶（3-MST）以及炎性因子TNF-α、NF-κB p50、COX-2和PTGES的mRNA表达。我们的结果表明，与对照组相比，喂食低硒日粮的鸡表现出组织学变化、较低的HS产生以及产生HS的酶（CSE、CBS和3-MST）的mRNA表达降低，而肠道炎性因子（TNF-α、NF-κB p50、COX-2和PTGES）的mRNA表达较高。我们的结果表明，低硒日粮可能会影响小肠中的HS、产生HS的酶和炎性因子，这意味着硒在维持肠道功能方面很重要，并且在硒缺乏诱导的炎症中HS产生下调。这种下调会加剧炎症并影响HS介导的肠道功能。
