College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, PR China.
College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, PR China.
Carbohydr Polym. 2016 Aug 20;147:354-364. doi: 10.1016/j.carbpol.2016.03.092. Epub 2016 Apr 1.
The optimal extraction conditions with a yield of 5.37±0.15% for extraction of polysaccharides from chickpea (Cicer arietinum L.) hull (CHPS) were determined as extraction temperature 99°C, extraction time 2.8h and ratio of water to raw material 24mL/g. Three fractions of CHPS-1, CHPS-2 and CHPS-3, with average molecular weight of 3.1×10(6), 1.5×10(6) and 7.8×10(5)Da, respectively, were obtained from crude CHPS by chromatography of DEAE Fast Flow and Sephadex G-100. CHPS-1 was composed of mannose, rhamnose, galactose, galacturonic acid, glucose and arabinose, CHPS-2 was composed of mannose, rhamnose, galacturonic acid, galactose, xylose and arabinose, CHPS-3 was composed of galacturonic acid, galactose and rhamnose. CHPS-3 showed the strongest reducing power and protective effect on H2O2-induced oxidative injury in PC12 cells and highest scavenging activities against DPPH and ABTS radicals, while CHPS-2 showed the highest scavenging activity against superoxide anion radical.
从鹰嘴豆壳（CHPS）中提取多糖的最佳提取条件为提取温度 99°C，提取时间 2.8h，水与原料比为 24mL/g，产率为 5.37±0.15%。通过 DEAE Fast Flow 和 Sephadex G-100 色谱法从粗 CHPS 中得到三种 CHPS-1、CHPS-2 和 CHPS-3 级分，平均分子量分别为 3.1×10(6)、1.5×10(6)和 7.8×10(5)Da。CHPS-1 由甘露糖、鼠李糖、半乳糖、半乳糖醛酸、葡萄糖和阿拉伯糖组成，CHPS-2 由甘露糖、鼠李糖、半乳糖醛酸、半乳糖、木糖和阿拉伯糖组成，CHPS-3 由半乳糖醛酸、半乳糖和鼠李糖组成。CHPS-3 具有最强的还原能力和对 H2O2 诱导的 PC12 细胞氧化损伤的保护作用，对 DPPH 和 ABTS 自由基的清除活性最高，而 CHPS-2 对超氧阴离子自由基的清除活性最高。
