Department of Microbiology and Biotechnology, Vilnius University, M. K. Čiurlionio Str. 21/27, Vilnius LT-03100, Lithuania.
Department of Microbiology and Biotechnology, Vilnius University, M. K. Čiurlionio Str. 21/27, Vilnius LT-03100, Lithuania.
Int J Biol Macromol. 2016 Aug;89:669-76. doi: 10.1016/j.ijbiomac.2016.05.041. Epub 2016 May 12.
There is a growing interest in supporting human health by using prebiotics, such as oligosaccharides, and beneficial bacteria, also called probiotics. Combining these two components we can develop synbiotics. In order to create successful combination of synbiotic it is very important to evaluate the influence of prebiotic oligosaccharides to probiotic bacteria and their behavior, such as growth and secretion of health related biomolecules, including bacteriocins. In this study seven type strains of probiotic bacteria (five Lactobacillus sp. and two Lactococcus sp.) and two Lactobacillus sp. strains, isolated from probiotic yoghurt, were cultivated with various commercially available and extracted oligosaccharides (OS). The aim of this study was to evaluate the influence of these OS on type and isolated bacterial strains growth and antibacterial activity. Obtained results suggest that combination of certain OS with probiotic strains may considerably improve their growth and/or antibacterial activity. We also determined the antibacterial activity spectrum of investigated strains with combination of OS against common food borne pathogens. Results of this work show that prebiotic OS can be useful for modulating probiotic bacteria growth, antibacterial activity and even specificity of this activity.
人们越来越关注通过使用益生元（如低聚糖）和有益细菌（也称为益生菌）来支持人类健康。将这两种成分结合起来，我们可以开发出合生元。为了成功地组合合生元，评估益生元低聚糖对益生菌的影响及其行为（如生长和分泌与健康相关的生物分子，包括细菌素）非常重要。在这项研究中，我们培养了七株益生菌（五种乳酸菌和两种乳球菌）和两株从益生菌酸奶中分离出来的乳酸菌菌株，使用各种市售和提取的低聚糖（OS）。本研究的目的是评估这些 OS 对类型和分离的细菌菌株生长和抗菌活性的影响。研究结果表明，某些 OS 与益生菌菌株的组合可以显著改善其生长和/或抗菌活性。我们还确定了 OS 与 OS 组合对常见食源性病原体的调查菌株的抗菌活性谱。这项工作的结果表明，益生元 OS 可用于调节益生菌的生长、抗菌活性，甚至调节这种活性的特异性。
