García-Gamboa Ricardo, Domínguez-Simi Miguel, Gradilla-Hernández Misael S, Bravo Jorge, Moya Andrés, Ruiz-Álvarez Blanca, González-Avila Marisela
Centre for Research and Assistance in Technology and Design of the State of Jalisco A.C., Normalistas 800, Guadalajara 44270, Mexico.
Tecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Av. General Ramón Corona 2514, Nuevo Mexico, Zapopan 45138, Mexico.
Antibiotics (Basel). 2022 Aug 21;11(8):1135. doi: 10.3390/antibiotics11081135.
There is great interest in the search for new alternatives to antimicrobial drugs, and the use of synbiotics is a promising approach to this problem. This study evaluated the growth inhibition and antibiofilm activity of the short-chain fatty acids produced by and in combination with inulin-type fructans against .
The growth inhibition of was evaluated using microdilution analysis in 96-well microtiter plates; different concentrations of cell-free supernatants of and were exposed to . The antibiofilm assessment was carried out using the crystal violet staining assay. The short-chain fatty acids were analyzed by gas chromatography.
The clinically isolated interacted with supernatants from and and showed significant growth inhibition and antibiofilm formation versus the controls. Lactate and acetic acid were elevated in the supernatants. The results suggest that the supernatants obtained from the synbiotic combinations of and with inulin-type fructans can inhibit the growth and biofilm formation against a clinically isolated strain.
These results suggest that synbiotic formulations could be a promising alternative to antifungal drugs in candidiasis therapy.
对抗菌药物新替代物的探索备受关注，而合生元的使用是解决这一问题的一种有前景的方法。本研究评估了[具体微生物1]和[具体微生物2]产生的短链脂肪酸与菊粉型果聚糖联合对[目标真菌]的生长抑制和抗生物膜活性。
在96孔微量滴定板中使用微量稀释分析法评估[目标真菌]的生长抑制；将不同浓度的[具体微生物1]和[具体微生物2]的无细胞上清液暴露于[目标真菌]。使用结晶紫染色法进行抗生物膜评估。通过气相色谱分析短链脂肪酸。
临床分离的[目标真菌]与[具体微生物1]和[具体微生物2]的上清液相互作用，与对照组相比显示出显著的生长抑制和生物膜形成抑制。上清液中乳酸和乙酸含量升高。结果表明，从[具体微生物1]和[具体微生物2]与菊粉型果聚糖的合生元组合中获得的上清液可抑制临床分离的[目标真菌]菌株的生长和生物膜形成。
这些结果表明，合生元制剂可能是念珠菌病治疗中抗真菌药物的一种有前景的替代物。
