Anticandidal and Antibiofilm Effect of Synbiotics including Probiotics and Inulin-Type Fructans.

BACKGROUND

There is great interest in the search for new alternatives to antimicrobial drugs, and the use of synbiotics is a promising approach to this problem. This study evaluated the growth inhibition and antibiofilm activity of the short-chain fatty acids produced by and in combination with inulin-type fructans against .

METHODS

The growth inhibition of was evaluated using microdilution analysis in 96-well microtiter plates; different concentrations of cell-free supernatants of and were exposed to . The antibiofilm assessment was carried out using the crystal violet staining assay. The short-chain fatty acids were analyzed by gas chromatography.

RESULTS

The clinically isolated interacted with supernatants from and and showed significant growth inhibition and antibiofilm formation versus the controls. Lactate and acetic acid were elevated in the supernatants. The results suggest that the supernatants obtained from the synbiotic combinations of and with inulin-type fructans can inhibit the growth and biofilm formation against a clinically isolated strain.

CONCLUSIONS

These results suggest that synbiotic formulations could be a promising alternative to antifungal drugs in candidiasis therapy.