Xu Pei, Du Peng-Xuan, Zong Min-Hua, Li Ning, Lou Wen-Yong
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
Laboratory of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Sci Rep. 2016 May 17;6:26158. doi: 10.1038/srep26158.
The efficient anti-Prelog asymmetric reduction of 2-octanone with Acetobacter pasteurianus GIM1.158 cells was successfully performed in a biphasic system consisting of deep eutectic solvent (DES) and water-immiscible ionic liquid (IL). Various DESs exerted different effects on the synthesis of (R)-2-octanol. Choline chloride/ethylene glycol (ChCl/EG) exhibited good biocompatibility and could moderately increase the cell membrane permeability thus leading to the better results. Adding ChCl/EG increased the optimal substrate concentration from 40 mM to 60 mM and the product e.e. kept above 99.9%. To further improve the reaction efficiency, water-immiscible ILs were introduced to the reaction system and an enhanced substrate concentration (1.5 M) was observed with C4MIM·PF6. Additionally, the cells manifested good operational stability in the reaction system. Thus, the efficient biocatalytic process with ChCl/EG and C4MIM·PF6 was promising for efficient synthesis of (R)-2-octanol.
利用巴氏醋酸杆菌GIM1.158细胞对2-辛酮进行高效的反普雷洛不对称还原反应，在由低共熔溶剂（DES）和与水不混溶的离子液体（IL）组成的双相体系中成功实现。各种DES对（R）-2-辛醇的合成表现出不同的影响。氯化胆碱/乙二醇（ChCl/EG）表现出良好的生物相容性，能适度提高细胞膜通透性，从而得到更好的结果。添加ChCl/EG使最佳底物浓度从40 mM提高到60 mM，产物对映体过量值（e.e.）保持在99.9%以上。为进一步提高反应效率，将与水不混溶的IL引入反应体系，使用C4MIM·PF6时观察到底物浓度提高到了1.5 M。此外，细胞在反应体系中表现出良好的操作稳定性。因此，ChCl/EG和C4MIM·PF6的高效生物催化过程有望用于高效合成（R）-2-辛醇。
