Li Xuan, Yang Zujing, Liao Huan, Zhang Zhengrui, Huang Xiaoting, Bao Zhenmin
Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China.
Comp Cytogenet. 2016 Mar 21;10(1):157-69. doi: 10.3897/CompCytogen.v10i1.7391. eCollection 2016.
Construction of cytogenetic maps can provide important information for chromosome identification, chromosome evolution and genomic research. However, it hasn't been conducted in many scallop species yet. In the present study, we attempted to map 12 fosmid clones containing tandem repeats by fluorescence in situ hybridization (FISH) in the Yesso scallop Patinopecten yessoensis (Jay, 1857). The results showed 6 fosmid clones were successfully mapped and distributed in 6 different pairs of chromosomes. Three clones were respectively assigned to a pair of metacentric chromosomes, a pair of submetacentric chromosomes and a pair of telocentric chromosomes and the remaining 3 clones showed their loci on three different pairs of subtelocentric chromosomes by co-hybridization. In summary, totally 8 pairs of chromosomes of the Yesso scallop were identified by 6 fosmid clones and two rDNA probes. Furthermore, 6 tandem repeats of 5 clones were sequenced and could be developed as chromosome specific markers for the Yesso scallop. The successful localization of fosmid clones will undoubtedly facilitate the integration of linkage groups with cytogenetic map and genomic research for the Yesso scallop.
细胞遗传图谱的构建可为染色体识别、染色体进化及基因组研究提供重要信息。然而，许多扇贝物种尚未开展此项工作。在本研究中，我们尝试通过荧光原位杂交（FISH）技术，将12个含有串联重复序列的fosmid克隆定位到虾夷扇贝（Patinopecten yessoensis，Jay，1857）的染色体上。结果显示，6个fosmid克隆成功定位并分布于6对不同的染色体上。通过共杂交，3个克隆分别定位到一对中着丝粒染色体、一对亚中着丝粒染色体和一对端着丝粒染色体上，其余3个克隆则在三对不同的亚端着丝粒染色体上显示出其位点。总之，利用6个fosmid克隆和两个rDNA探针共鉴定出了虾夷扇贝的8对染色体。此外，对5个克隆的6个串联重复序列进行了测序，可将其开发为虾夷扇贝的染色体特异性标记。fosmid克隆的成功定位无疑将有助于虾夷扇贝连锁群与细胞遗传图谱的整合及基因组研究。
