Yang Zujing, Li Xuan, Liao Huan, Hu Liping, Zhang Zhengrui, Zhao Bosong, Huang Xiaoting, Bao Zhenmin
Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China.
Yantai Fisheries Research Institute, Yantai 264003, China.
Comp Cytogenet. 2016 Oct 25;10(4):529-541. doi: 10.3897/CompCytogen.v10i4.10047. eCollection 2016.
The innate immune system plays a pivotal role in defending invasion of microorganisms for scallops. Previous studies on immune-related genes in the Yesso scallop, (Jay, 1857) have mainly focused on characterization and expression pattern in response to bacterial challenge, no research has been carried out on the cytogenetic level yet. In the present study, eight fosmid clones containing the sequences of key immune-related genes (NFkB, TRAF2, TRAF4, TRAF7, Myd88-1, Myd88-3, MKK-7 and TNFR) were isolated and seven of them were successfully mapped on chromosomes of utilizing fluorescence hybridization. Wherein, Myd88-1, Myd88-3 and MKK-7 located on the same chromosome pair with adjacent positions and the other genes were mapped on four non-homologous chromosome pairs, showing a similar distribution to another five model species. The isolation and mapping of such genes of the Yesso scallop will lay a foundation for studies such as assignment of interested genes to chromosomes, construction cytogenetic maps and so on.
先天免疫系统在扇贝抵御微生物入侵中起关键作用。此前对虾夷扇贝（Jay，1857）免疫相关基因的研究主要集中在其对细菌攻击的特征描述及表达模式上，尚未在细胞遗传学水平开展研究。在本研究中，分离出了八个包含关键免疫相关基因（NFkB、TRAF2、TRAF4、TRAF7、Myd88 - 1、Myd88 - 3、MKK - 7和TNFR）序列的fosmid克隆，其中七个通过荧光原位杂交成功定位到虾夷扇贝的染色体上。其中，Myd88 - 1、Myd88 - 3和MKK - 7位于同一染色体对的相邻位置，其他基因定位在四个非同源染色体对上，与另外五个模式物种的分布相似。虾夷扇贝此类基因的分离和定位将为将感兴趣的基因定位到染色体、构建细胞遗传图谱等研究奠定基础。
