抽搐运动性与环磷酸腺苷水平：通过单一甲基接受趋化蛋白的信号转导
Twitching motility and cAMP levels: signal transduction through a single methyl-accepting chemotaxis protein.
作者信息
Jansari Vibhuti H, Potharla Vishwakanth Y, Riddell Geoff T, Bardy Sonia L
机构信息
Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA.
Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA
出版信息
FEMS Microbiol Lett. 2016 Jun;363(12). doi: 10.1093/femsle/fnw119. Epub 2016 May 1.
Abstract
The Pseudomonas aeruginosa Chp chemosensory system regulates twitching motility, intracellular adenosine 3('') 5(')-cyclic monophosphate (cAMP) levels and is postulated to be involved in directional twitching towards phosphatidylethanolamine (PE). Because PilJ is the only methyl-accepting chemotaxis protein (MCP) identified in the Chp system, we determined the role of PilJ in mediating signal transduction for the distinct outputs of this system. Mutants that lack the periplasmic domain of PilJ (pilJΔ74-273) showed lower levels of cAMP but retained directional twitching towards PE. While initial studies revealed reduced twitching motility by PilJΔ74-273, this was due to decreased cAMP levels. Our data illustrate the importance of the periplasmic domain of PilJ in regulating cAMP. This is the first time a defined domain within PilJ has been identified as having a distinct role in signal transduction.
摘要
铜绿假单胞菌Chp化学感应系统调节颤动运动性、细胞内3('') 5(')-环磷酸腺苷（cAMP）水平，并据推测参与向磷脂酰乙醇胺（PE）的定向颤动。由于PilJ是在Chp系统中鉴定出的唯一甲基接受趋化蛋白（MCP），我们确定了PilJ在介导该系统不同输出的信号转导中的作用。缺乏PilJ周质结构域的突变体（pilJΔ74-273）显示cAMP水平较低，但仍保留对PE的定向颤动。虽然初步研究表明PilJΔ74-273的颤动运动性降低，但这是由于cAMP水平降低所致。我们的数据说明了PilJ周质结构域在调节cAMP中的重要性。这是首次确定PilJ内的一个特定结构域在信号转导中具有独特作用。
相似文献
1
Twitching motility and cAMP levels: signal transduction through a single methyl-accepting chemotaxis protein.抽搐运动性与环磷酸腺苷水平：通过单一甲基接受趋化蛋白的信号转导
FEMS Microbiol Lett. 2016 Jun;363(12). doi: 10.1093/femsle/fnw119. Epub 2016 May 1.
2
ChpC controls twitching motility-mediated expansion of biofilms in response to serum albumin, mucin and oligopeptides.ChpC 控制着生物膜在血清白蛋白、粘蛋白和寡肽作用下的抽搐运动介导的扩张。
Microbiology (Reading). 2020 Jul;166(7):669-678. doi: 10.1099/mic.0.000911.
3
Cyclic AMP-Independent Control of Twitching Motility in Pseudomonas aeruginosa.铜绿假单胞菌中抽动运动的环磷酸腺苷非依赖性调控
J Bacteriol. 2017 Jul 25;199(16). doi: 10.1128/JB.00188-17. Print 2017 Aug 15.
4
PilJ localizes to cell poles and is required for type IV pilus extension in Pseudomonas aeruginosa.菌毛蛋白J定位于细胞极，是铜绿假单胞菌IV型菌毛延伸所必需的。
Curr Microbiol. 2007 Nov;55(5):389-95. doi: 10.1007/s00284-007-9008-5. Epub 2007 Aug 23.
5
Characterization of a Pseudomonas aeruginosa gene cluster involved in pilus biosynthesis and twitching motility: sequence similarity to the chemotaxis proteins of enterics and the gliding bacterium Myxococcus xanthus.参与菌毛生物合成和颤动运动的铜绿假单胞菌基因簇的特征：与肠道菌及滑行细菌粘球菌趋化蛋白的序列相似性
Mol Microbiol. 1994 Jan;11(1):137-53. doi: 10.1111/j.1365-2958.1994.tb00296.x.
6
Type IV pili mechanochemically regulate virulence factors in Pseudomonas aeruginosa.IV型菌毛通过机械化学方式调节铜绿假单胞菌中的毒力因子。
Proc Natl Acad Sci U S A. 2015 Jun 16;112(24):7563-8. doi: 10.1073/pnas.1502025112. Epub 2015 Jun 3.
7
The Pseudomonas aeruginosa Chp chemosensory system regulates intracellular cAMP levels by modulating adenylate cyclase activity.铜绿假单胞菌 Chp 化学感觉系统通过调节腺苷酸环化酶活性来调节细胞内 cAMP 水平。
Mol Microbiol. 2010 May;76(4):889-904. doi: 10.1111/j.1365-2958.2010.07135.x. Epub 2010 Mar 16.
8
Mechanotaxis directs twitching motility.机械趋性指导菌毛的抖动运动。
Proc Natl Acad Sci U S A. 2021 Jul 27;118(30). doi: 10.1073/pnas.2101759118.
9
Characterization of a complex chemosensory signal transduction system which controls twitching motility in Pseudomonas aeruginosa.一种控制铜绿假单胞菌抽搐运动性的复杂化学感应信号转导系统的特性分析。
Mol Microbiol. 2004 May;52(3):873-93. doi: 10.1111/j.1365-2958.2004.04026.x.
10
Extragenic suppressor mutations that restore twitching motility to fimL mutants of Pseudomonas aeruginosa are associated with elevated intracellular cyclic AMP levels.外基因抑制突变可恢复铜绿假单胞菌 fimL 突变体的蠕动运动，与细胞内环腺苷酸水平升高有关。
Microbiologyopen. 2012 Dec;1(4):490-501. doi: 10.1002/mbo3.49. Epub 2012 Nov 28.
引用本文的文献
1
Functional investigation of Zur in metal ion homeostasis, motility and multiple stresses resistance in cyanobacteria Synechocystis sp. PCC 6803.集胞藻6803中Zur在金属离子稳态、运动性和多重胁迫抗性方面的功能研究
Stress Biol. 2025 May 7;5(1):32. doi: 10.1007/s44154-025-00224-x.
2
Structural conservation and functional role of TfpY-like proteins in type IV pilus assembly.TfpY样蛋白在IV型菌毛组装中的结构保守性和功能作用
J Bacteriol. 2025 Feb 20;207(2):e0034324. doi: 10.1128/jb.00343-24. Epub 2025 Jan 16.
3
A bacterial sense of touch: T4P retraction motor as a means of surface sensing by PA14.细菌的触觉：T4P 回缩马达作为 PA14 表面感应的一种手段。
J Bacteriol. 2024 Jul 25;206(7):e0044223. doi: 10.1128/jb.00442-23. Epub 2024 Jun 4.
4
The type IV pilus chemoreceptor PilJ controls chemotaxis of one bacterial species towards another.IV 型菌毛化学感受器 PilJ 控制一种细菌对另一种细菌的趋化性。
PLoS Biol. 2024 Feb 13;22(2):e3002488. doi: 10.1371/journal.pbio.3002488. eCollection 2024 Feb.
5
Evidence for the Type IV Pilus Retraction Motor PilT as a Component of the Surface Sensing System in Pseudomonas aeruginosa.证据表明，Ⅳ型菌毛回缩马达蛋白 PilT 是铜绿假单胞菌表面感应系统的一个组成部分。
J Bacteriol. 2023 Jul 25;205(7):e0017923. doi: 10.1128/jb.00179-23. Epub 2023 Jun 29.
6
Evidence for the Type IV Pili Retraction Motor PilT as a Component of the Surface Sensing System in .IV型菌毛收缩马达蛋白PilT作为[具体生物]表面传感系统组成部分的证据。 （注：原文中“in.”后面缺少具体内容）
bioRxiv. 2023 May 2:2023.05.02.539127. doi: 10.1101/2023.05.02.539127.
7
A bacterial chemoreceptor that mediates chemotaxis to two different plant hormones.一种细菌化学感受器，介导对两种不同植物激素的趋化作用。
Environ Microbiol. 2022 Aug;24(8):3580-3597. doi: 10.1111/1462-2920.15920. Epub 2022 Feb 1.
8
as a Model To Study Chemosensory Pathway Signaling.作为研究化学感觉通路信号转导的模型。
Microbiol Mol Biol Rev. 2021 Jan 13;85(1). doi: 10.1128/MMBR.00151-20. Print 2021 Feb 17.
9
The Molecular Mechanism of Nitrate Chemotaxis via Direct Ligand Binding to the PilJ Domain of McpN.通过直接配体结合到 McpN 的 PilJ 结构域实现硝酸盐化学趋性的分子机制。
mBio. 2019 Feb 19;10(1):e02334-18. doi: 10.1128/mBio.02334-18.
10
Cyclic AMP-Independent Control of Twitching Motility in Pseudomonas aeruginosa.铜绿假单胞菌中抽动运动的环磷酸腺苷非依赖性调控
J Bacteriol. 2017 Jul 25;199(16). doi: 10.1128/JB.00188-17. Print 2017 Aug 15.
本文引用的文献
1
Type IV pili mechanochemically regulate virulence factors in Pseudomonas aeruginosa.IV型菌毛通过机械化学方式调节铜绿假单胞菌中的毒力因子。
Proc Natl Acad Sci U S A. 2015 Jun 16;112(24):7563-8. doi: 10.1073/pnas.1502025112. Epub 2015 Jun 3.
2
A hierarchical cascade of second messengers regulates Pseudomonas aeruginosa surface behaviors.第二信使的分级级联反应调节铜绿假单胞菌的表面行为。
mBio. 2015 Jan 27;6(1):e02456-14. doi: 10.1128/mBio.02456-14.
3
Pseudomonas aeruginosa twitching motility: type IV pili in action.铜绿假单胞菌的菌毛运动：IV 型菌毛的作用。
Annu Rev Microbiol. 2012;66:493-520. doi: 10.1146/annurev-micro-092611-150055. Epub 2012 Jul 2.
4
Crystal structure and regulation mechanisms of the CyaB adenylyl cyclase from the human pathogen Pseudomonas aeruginosa.铜绿假单胞菌 CyaB 腺苷酸环化酶的晶体结构与调控机制。
J Mol Biol. 2012 Feb 17;416(2):271-86. doi: 10.1016/j.jmb.2011.12.045. Epub 2011 Dec 28.
5
Phenol sensing by Escherichia coli chemoreceptors: a nonclassical mechanism.大肠杆菌化学感受器对苯酚的感应：一种非经典机制。
J Bacteriol. 2011 Dec;193(23):6597-604. doi: 10.1128/JB.05987-11. Epub 2011 Sep 30.
6
Key two-component regulatory systems that control biofilm formation in Pseudomonas aeruginosa.控制铜绿假单胞菌生物膜形成的两个关键组成部分调控系统。
Environ Microbiol. 2011 Jul;13(7):1666-81. doi: 10.1111/j.1462-2920.2011.02495.x. Epub 2011 May 9.
7
Signaling mechanisms of HAMP domains in chemoreceptors and sensor kinases.化学感受器和传感器激酶中 HAMP 结构域的信号转导机制。
Annu Rev Microbiol. 2010;64:101-22. doi: 10.1146/annurev.micro.112408.134215.
8
The Pseudomonas aeruginosa Chp chemosensory system regulates intracellular cAMP levels by modulating adenylate cyclase activity.铜绿假单胞菌 Chp 化学感觉系统通过调节腺苷酸环化酶活性来调节细胞内 cAMP 水平。
Mol Microbiol. 2010 May;76(4):889-904. doi: 10.1111/j.1365-2958.2010.07135.x. Epub 2010 Mar 16.
9
Increase in rhamnolipid synthesis under iron-limiting conditions influences surface motility and biofilm formation in Pseudomonas aeruginosa.在缺铁条件下，鼠李糖脂的合成增加会影响铜绿假单胞菌的表面运动性和生物膜形成。
J Bacteriol. 2010 Jun;192(12):2973-80. doi: 10.1128/JB.01601-09. Epub 2010 Feb 12.
10
Genetic analysis of the regulation of type IV pilus function by the Chp chemosensory system of Pseudomonas aeruginosa.铜绿假单胞菌 Chp 化感系统对 IV 型菌毛功能调控的遗传分析。
J Bacteriol. 2010 Feb;192(4):994-1010. doi: 10.1128/JB.01390-09. Epub 2009 Dec 11.
Zotero 插件