臭氧疗法在实验性葡萄膜炎大鼠模型中的疗效。

Efficiency of Ozone Therapy in a Rat Model of Experimental Uveitis.

机构信息

a Department of Ophthalmology , Anıttepe Military Dispansery , Ankara , Turkey.

b Department of Ophthalmology , GATA Haydarpasa Training Hospital , Istanbul , Turkey.

出版信息

Ocul Immunol Inflamm. 2017 Oct;25(5):695-700. doi: 10.3109/09273948.2016.1161057. Epub 2016 May 18.

DOI:10.3109/09273948.2016.1161057

PMID:27192545

Abstract

PURPOSE

To investigate efficiency of ozone therapy in uveitis.

METHODS

A total of 24 albino Wistar rats were randomly assigned to four groups (n = 6); (a) control group; (b) sham group; (c) infliximab treatment group; (d) ozone therapy group. Vitreous haze scores of all groups were evaluated. Vitreous cytokine levels (TNF-α, IL-1, IL-6) measured by ELISA and eyes were enucleated for histopathologic examination.

RESULTS

According to vitreous haze scores, there was statistically significant inflammation in Group (b) compared with Group (a), and there was less inflammation in infliximab and ozone groups compared with Group (b) (p < 0.05). Cytokine levels in infliximab and ozone groups were lower but not statistically significant when compared with Group (b) (p > 0.05). There was significantly less inflammation in histopathologic examination in treatment groups when compared with the sham group (p < 0.05).

CONCLUSIONS

Clinical and histopathologic examination results indicate that systemic application of ozone may be efficient in the treatment of uveitis.

摘要

目的

研究臭氧治疗在葡萄膜炎中的疗效。

方法

将 24 只白化 Wistar 大鼠随机分为四组（n = 6）；（a）对照组；（b）假手术组；（c）英夫利昔单抗治疗组；（d）臭氧治疗组。评估所有组的玻璃体细胞混浊评分。通过 ELISA 测量玻璃体内细胞因子（TNF-α、IL-1、IL-6）水平，并对眼球进行组织病理学检查。

结果

根据玻璃体细胞混浊评分，与组（a）相比，组（b）有统计学意义的炎症，与组（b）相比，英夫利昔单抗和臭氧组的炎症较轻（p < 0.05）。与组（b）相比，英夫利昔单抗和臭氧组的细胞因子水平较低，但无统计学意义（p > 0.05）。与假手术组相比，治疗组的组织病理学检查炎症明显减轻（p < 0.05）。