Ribeiro Aline Rimoldi, Oliveira Rosane Correa de, Ceretti Junior Walter, Lima Luciana, Almeida Larissa Aguiar de, Nascimento Juliana Damieli, Teixeira Marta Maria Geraldes, Rosa João Aristeu da
Departamento de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.
Secretaria Municipal de Saúde de São Paulo, São Paulo, Brazil.
Rev Soc Bras Med Trop. 2016 Apr;49(2):183-9. doi: 10.1590/0037-8682-0366-2015.
To characterize Trypanosoma cruzi (TcI) isolated from a Panstrongylus megistus specimen found in one of the biggest metropolitan areas of Latin America, the relationship between the TcI group of T. cruzi and the transmission cycle in the urban environment was studied.
The T. cruzi strain, Pm, was isolated in a culture medium from the evolutionary forms present in the hindgut of a live male specimen of P. megistus found in the Jabaquara subway in São Paulo City. The sample from the triatomine showed trypomastigote forms of Trypanosomatidae, which were inoculated in the peritoneum of Balb/c mice. The sample was then inoculated in Liver Infusion Tryptose medium and J774 cells for the molecular identification and characterization of the parasite. The Pm strain of T. cruzi was identified by isolation in axenic culture medium, and based on the morphology, cell infection, growth kinetics, and molecular characterization.
After isolation, the protozoan was identified as T. cruzi. No parasites were detected in the peripheral blood of the animal, which can be a characteristic inherent to the strain of T. cruzi that was isolated. Cell invasion assays were performed in triplicate in the J774 cell line to confirm the invasive ability of the Pm strain and revealed amastigote forms of the parasite within macrophages.
Our biological and molecular characterizations helped understand parasite-host interactions and their evolutionary history in context of the associations between vectors, ecotopes, hosts, and groups of the parasite.
为了对从拉丁美洲最大的大都市区之一发现的一只大锥蝽标本中分离出的克氏锥虫（TcI）进行特征描述，研究了克氏锥虫TcI组与城市环境中传播周期之间的关系。
从圣保罗市雅巴夸拉地铁站发现的一只活的雄性大锥蝽标本后肠中存在的进化形态，在培养基中分离出克氏锥虫菌株Pm。来自锥蝽的样本显示出锥虫科的锥鞭毛体形态，将其接种到Balb/c小鼠的腹腔中。然后将该样本接种到肝浸液胰蛋白胨培养基和J774细胞中，用于寄生虫的分子鉴定和特征描述。通过在无细胞培养基中分离，并基于形态学、细胞感染、生长动力学和分子特征，鉴定了克氏锥虫的Pm菌株。
分离后，原生动物被鉴定为克氏锥虫。在动物外周血中未检测到寄生虫，这可能是所分离的克氏锥虫菌株固有的特征。在J774细胞系中进行了三次重复的细胞侵袭试验，以确认Pm菌株的侵袭能力，并在巨噬细胞内发现了寄生虫的无鞭毛体形态。
我们的生物学和分子特征描述有助于在媒介、生态位、宿主和寄生虫群体之间的关联背景下理解寄生虫与宿主的相互作用及其进化历史。
