Bobko Andrey A, Evans Jason, Denko Nicholas C, Khramtsov Valery V
In Vivo Multifunctional Magnetic Resonance Center, Robert C. Byrd Health Science Center, West Virginia University, Morgantown, WV, 26506, USA.
Department of Biochemistry, West Virginia University School of Medicine, Morgantown, WV, 26506, USA.
Cell Biochem Biophys. 2017 Jun;75(2):247-253. doi: 10.1007/s12013-016-0733-x. Epub 2016 May 18.
Tissue oxygenation, extracellular acidity, and tissue reducing capacity are among crucial parameters of tumor microenvironment (TME) of significant importance for tumor pathophysiology. In this paper, we demonstrate the complementary application of particulate lithium octa-n-butoxy-naphthalocyanine and soluble nitroxide paramagnetic probes for monitoring of these TME parameters using electron paramagnetic resonance (EPR) technique. Two different types of therapeutic interventions were studied: hypothermia and systemic administration of metabolically active drug. In summary, the results demonstrate the utility of EPR technique for non-invasive concurrent longitudinal monitoring of physiologically relevant chemical parameters of TME in mouse xenograft tumor models, including that under therapeutic intervention.
组织氧合、细胞外酸度和组织还原能力是肿瘤微环境（TME）的关键参数，对肿瘤病理生理学具有重要意义。在本文中，我们展示了颗粒状八正丁氧基萘酞菁锂和可溶性氮氧化物顺磁探针的互补应用，用于使用电子顺磁共振（EPR）技术监测这些TME参数。研究了两种不同类型的治疗干预：低温和代谢活性药物的全身给药。总之，结果证明了EPR技术在小鼠异种移植肿瘤模型中对TME生理相关化学参数进行非侵入性同步纵向监测的实用性，包括治疗干预下的参数监测。
