Bobko Andrey A, Eubank Timothy D, Driesschaert Benoit, Khramtsov Valery V
In Vivo Multifunctional Magnetic Resonance center, Robert C. Byrd Health Sciences Center, West Virginia University; Department of Biochemistry, West Virginia University School of Medicine.
In Vivo Multifunctional Magnetic Resonance center, Robert C. Byrd Health Sciences Center, West Virginia University; Department of Microbiology, Immunology & Cell Biology, West Virginia University School of Medicine.
J Vis Exp. 2018 Mar 16(133):56624. doi: 10.3791/56624.
This protocol demonstrates the capability of low-field electron paramagnetic resonance (EPR)-based techniques in combination with functional paramagnetic probes to provide quantitative information on the chemical tumor microenvironment (TME), including pO2, pH, redox status, concentrations of interstitial inorganic phosphate (Pi), and intracellular glutathione (GSH). In particular, an application of a recently developed soluble multifunctional trityl probe provides unsurpassed opportunity for in vivo concurrent measurements of pH, pO2 and Pi in Extracellular space (HOPE probe). The measurements of three parameters using a single probe allow for their correlation analyses independent of probe distribution and time of the measurements.
本方案展示了基于低场电子顺磁共振（EPR）的技术与功能性顺磁探针相结合，提供关于化学肿瘤微环境（TME）定量信息的能力，这些信息包括氧分压（pO2）、酸碱度（pH）、氧化还原状态、间质无机磷酸盐（Pi）浓度和细胞内谷胱甘肽（GSH）。特别是，最近开发的一种可溶性多功能三苯甲基探针的应用，为在细胞外空间同时进行pH、pO2和Pi的体内测量提供了无与伦比的机会（HOPE探针）。使用单一探针测量这三个参数，可对它们进行相关性分析，而不受探针分布和测量时间的影响。
