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1
Effects of antibody to 5 S-RNA-binding protein on protein synthesis in Artemia salina ribosomes.抗5 S - RNA结合蛋白抗体对卤虫核糖体蛋白质合成的影响。
Biochem J. 1989 Apr 1;259(1):277-81. doi: 10.1042/bj2590277.
2
Changes in ribosomal proteins in developing Artemia salina embryos.卤虫胚胎发育过程中核糖体蛋白的变化。
J Biochem. 1989 Aug;106(2):289-93. doi: 10.1093/oxfordjournals.jbchem.a122846.
3
Probing ribosome function and the location of Escherichia coli ribosomal protein L5 with a monoclonal antibody.用单克隆抗体探究核糖体功能及大肠杆菌核糖体蛋白L5的定位
J Biol Chem. 1987 Jul 15;262(20):9681-7.
4
In vitro incorporation of eubacterial, archaebacterial and eukaryotic 5S rRNAs into large ribosomal subunits of Bacillus stearothermophilus.嗜热脂肪芽孢杆菌大核糖体亚基中外源真细菌、古细菌和真核生物5S rRNA的体外掺入
Nucleic Acids Res. 1988 Apr 25;16(8):3511-24. doi: 10.1093/nar/16.8.3511.
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An argument for the existence of a natural complex between protein L5 and 5 S RNA in rat liver 60-S ribosomal subunits.关于大鼠肝脏60-S核糖体亚基中蛋白质L5与5 S RNA之间存在天然复合物的论点。
Biochim Biophys Acta. 1981 Jan 29;652(1):193-203. doi: 10.1016/0005-2787(81)90222-7.
6
Synergistic defect in 60S ribosomal subunit assembly caused by a mutation of Rrs1p, a ribosomal protein L11-binding protein, and 3'-extension of 5S rRNA in Saccharomyces cerevisiae.酿酒酵母中核糖体蛋白L11结合蛋白Rrs1p的突变以及5S rRNA的3'端延伸导致60S核糖体亚基组装的协同缺陷。
Nucleic Acids Res. 2005 Aug 12;33(14):4553-62. doi: 10.1093/nar/gki772. Print 2005.
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Structural and functional exchangeability of 5 S RNA species from the eubacterium E.coli and the thermoacidophilic archaebacterium Sulfolobus solfataricus.来自真细菌大肠杆菌和嗜热嗜酸古细菌嗜热栖热菌的5S RNA种类的结构与功能互换性。
Nucleic Acids Res. 1989 Feb 11;17(3):845-51. doi: 10.1093/nar/17.3.845.
8
Probing the rRNA environment of ribosomal protein S5 across the subunit interface and inside the 30 S subunit using tethered Fe(II).利用 tethered Fe(II) 探究核糖体蛋白 S5 在亚基界面及 30 S 亚基内部的 rRNA 环境。
J Mol Biol. 1999 Feb 19;286(2):355-64. doi: 10.1006/jmbi.1998.2483.
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Interaction of yeast elongation factor 3 with polynucleotides, ribosomal RNA and ribosomal subunits.酵母延伸因子3与多核苷酸、核糖体RNA及核糖体亚基的相互作用。
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A difference in the importance of bulged nucleotides and their parent base pairs in the binding of transcription factor IIIA to Xenopus 5S RNA and 5S RNA genes.转录因子IIIA与非洲爪蟾5S RNA及5S RNA基因结合时，凸起核苷酸及其亲本碱基对的重要性差异。
Nucleic Acids Res. 1989 Mar 11;17(5):2043-56. doi: 10.1093/nar/17.5.2043.

本文引用的文献

1
CHARACTERIZATION OF A RIBOSOME-LINKED GUANOSINE TRIPHOSPHATASE IN ESCHERICHIA COLI EXTRACTS.大肠杆菌提取物中一种核糖体连接的鸟苷三磷酸酶的特性分析
Proc Natl Acad Sci U S A. 1964 Dec;52(6):1462-9. doi: 10.1073/pnas.52.6.1462.
2
Ribosomal proteins cross-linked to natural mRNA by UV irradiation of rat liver polysomes.通过紫外线照射大鼠肝脏多核糖体使核糖体蛋白与天然信使核糖核酸交联。
J Biochem. 1981 Nov;90(5):1549-52. doi: 10.1093/oxfordjournals.jbchem.a133624.
3
Isolation of a 5S RNA-protein L5 complex from 60S subunits of rat liver ribosomes by cesium sulfate density-gradient equilibrium centrifugation.通过硫酸铯密度梯度平衡离心从大鼠肝脏核糖体的60S亚基中分离出5S RNA-蛋白质L5复合物。
J Biochem. 1981 Aug;90(2):551-4. doi: 10.1093/oxfordjournals.jbchem.a133504.
4
Analyses of 40S and 60S ribosomal proteins of Artemia salina with two- or "three-dimensional" acrylamide gel electrophoresis and comparisons with rat liver 40S and 60S proteins.利用二维或“三维”丙烯酰胺凝胶电泳分析卤虫的40S和60S核糖体蛋白，并与大鼠肝脏的40S和60S蛋白进行比较。
J Biochem. 1981 Apr;89(4):1293-308.
5
Effects of ethionine treatment of protein-synthesizing apparatus of rat liver 80 S ribosomes and 40 S ribosomal subunits.乙硫氨酸处理对大鼠肝脏80S核糖体和40S核糖体亚基蛋白质合成装置的影响。
Biochim Biophys Acta. 1982 Apr 26;697(1):101-12. doi: 10.1016/0167-4781(82)90050-1.
6
Enzyme immunoassay ELISA and EMIT.酶免疫测定法（ELISA）和酶放大免疫测定技术（EMIT）。
Methods Enzymol. 1980;70(A):419-39. doi: 10.1016/s0076-6879(80)70067-8.
7
Identification of proteins at the peptidyl-tRNA binding site of rat liver ribosomes.大鼠肝脏核糖体肽基 - tRNA结合位点蛋白质的鉴定
Mol Gen Genet. 1981;184(3):551-6. doi: 10.1007/BF00352539.
8
An argument for the existence of a natural complex between protein L5 and 5 S RNA in rat liver 60-S ribosomal subunits.关于大鼠肝脏60-S核糖体亚基中蛋白质L5与5 S RNA之间存在天然复合物的论点。
Biochim Biophys Acta. 1981 Jan 29;652(1):193-203. doi: 10.1016/0005-2787(81)90222-7.
9
Location of eight ribosomal proteins on the surface of the 50S subunit from Escherichia coli.8种核糖体蛋白在大肠杆菌50S亚基表面的定位
Proc Natl Acad Sci U S A. 1983 Nov;80(22):6780-4. doi: 10.1073/pnas.80.22.6780.
10
Architecture of prokaryotic ribosomes.原核生物核糖体的结构
Annu Rev Biochem. 1983;52:35-65. doi: 10.1146/annurev.bi.52.070183.000343.

抗5 S - RNA结合蛋白抗体对卤虫核糖体蛋白质合成的影响。

Effects of antibody to 5 S-RNA-binding protein on protein synthesis in Artemia salina ribosomes.

作者信息

Kenmochi N, Takahashi Y, Sato N L

机构信息

Facility for Comparative Medicine, Niigata University School of Medicine, Japan.

出版信息

Biochem J. 1989 Apr 1;259(1):277-81. doi: 10.1042/bj2590277.

DOI:10.1042/bj2590277
PMID:2719645
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1138501/
Abstract

The effects of an affinity-purified polyclonal antibody to Artemia salina ribosomal protein L5 on protein synthesis in vitro were examined. The antibody interacted with 60 S subunits more strongly than with 80 S ribosomes, and inhibited reassociation of ribosomal subunits to some extent at 5 mM-Mg2+ but not at 10 mM. Polyphenylalanine synthesis in vitro at 10 mM-Mg2+ was significantly inhibited, especially when the antibody was first preincubated with 60 S subunits prior to the assay. The incorporation of amino acid directed by globin mRNA was inhibited only when the preincubation with 60 S subunits was carried out. On the other hand, no effect was detected on elongation factor 2- and 60 S subunit-dependent uncoupled GTPase activity. These results suggest that L5 is probably located at or near the subunit interface and may play an important role in protein synthesis.

摘要

研究了一种亲和纯化的针对卤虫核糖体蛋白L5的多克隆抗体对体外蛋白质合成的影响。该抗体与60 S亚基的相互作用比与80 S核糖体的相互作用更强，并且在5 mM - Mg2+ 时能在一定程度上抑制核糖体亚基的重新结合，但在10 mM时则不能。在10 mM - Mg2+ 条件下体外多聚苯丙氨酸的合成受到显著抑制，尤其是在测定前抗体先与60 S亚基预温育时。只有在与60 S亚基进行预温育时，球蛋白mRNA指导的氨基酸掺入才会受到抑制。另一方面，未检测到对延伸因子2和60 S亚基依赖性非偶联GTP酶活性有影响。这些结果表明，L5可能位于亚基界面处或其附近，并且可能在蛋白质合成中起重要作用。