酿酒酵母中核糖体蛋白L11结合蛋白Rrs1p的突变以及5S rRNA的3'端延伸导致60S核糖体亚基组装的协同缺陷。

Synergistic defect in 60S ribosomal subunit assembly caused by a mutation of Rrs1p, a ribosomal protein L11-binding protein, and 3'-extension of 5S rRNA in Saccharomyces cerevisiae.

作者信息

Nariai Masanobu, Tanaka Tomohisa, Okada Takafumi, Shirai Chiharu, Horigome Chihiro, Mizuta Keiko

机构信息

Department of Bioresource Science and Technology, Graduate School of Biosphere Science, Hiroshima University Kagamiyama, Higashi-Hiroshima 739-8528, Japan.

出版信息

Nucleic Acids Res. 2005 Aug 12;33(14):4553-62. doi: 10.1093/nar/gki772. Print 2005.

Rrs1p, a ribosomal protein L11-binding protein, has an essential role in biogenesis of 60S ribosomal subunits. We obtained conditionally synthetic lethal allele with the rrs1-5 mutation and determined that the mutation is in REX1, which encodes an exonuclease. The highly conserved leucine at 305 was substituted with tryptophan in rex1-1. The rex1-1 allele resulted in 3'-extended 5S rRNA. Polysome analysis revealed that rex1-1 and rrs1-5 caused a synergistic defect in the assembly of 60S ribosomal subunits. In vivo and in vitro binding assays indicate that Rrs1p interacts with the ribosomal protein L5-5S rRNA complex. The rrs1-5 mutation weakens the interaction between Rrs1p with both L5 and L11. These data suggest that the assembly of L5-5S rRNA on 60S ribosomal subunits coordinates with assembly of L11 via Rrs1p.

Rrs1p是一种核糖体蛋白L11结合蛋白，在60S核糖体亚基的生物合成中起关键作用。我们获得了与rrs1 - 5突变相关的条件性合成致死等位基因，并确定该突变位于REX1中，REX1编码一种核酸外切酶。在rex1 - 1中，305位高度保守的亮氨酸被色氨酸取代。rex1 - 1等位基因导致5S rRNA 3'端延长。多核糖体分析表明，rex1 - 1和rrs1 - 5在60S核糖体亚基组装中导致协同缺陷。体内和体外结合试验表明，Rrs1p与核糖体蛋白L5 - 5S rRNA复合物相互作用。rrs1 - 5突变削弱了Rrs1p与L5和L11之间的相互作用。这些数据表明，60S核糖体亚基上L5 - 5S rRNA的组装通过Rrs1p与L11的组装相协调。