嗜热脂肪芽孢杆菌大核糖体亚基中外源真细菌、古细菌和真核生物5S rRNA的体外掺入
In vitro incorporation of eubacterial, archaebacterial and eukaryotic 5S rRNAs into large ribosomal subunits of Bacillus stearothermophilus.
作者信息
Hartmann R K, Vogel D W, Walker R T, Erdmann V A
机构信息
Institut für Biochemie, Freie Universität Berlin, FRG.
出版信息
Nucleic Acids Res. 1988 Apr 25;16(8):3511-24. doi: 10.1093/nar/16.8.3511.
Abstract
Bacillus stearothermophilus large ribosomal subunits were reconstituted in the presence of 5S rRNAs from different origins and tested for their biological activities. The results obtained have shown that eubacterial and archaebacterial 5S rRNAs can easily substitute for B. stearothermophilus 5S rRNA in the reconstitution, while eukaryotic 5S rRNAs yield ribosomal subunits with reduced biological activities. From our results we propose an interaction between nucleotides 42-47 of 5S rRNA and nucleotides 2603-2608 of 23S rRNA during the assembly of the 50S ribosomal subunit. Other experiments with eukaryotic 5.8S rRNAs reveal, if at all, a very low incorporation of these RNA species into the reconstituted ribosomes.
摘要
嗜热栖热放线菌大核糖体亚基在来自不同来源的5S rRNA存在下进行了重组，并对其生物学活性进行了测试。所得结果表明，真细菌和古细菌的5S rRNA在重组过程中可以很容易地替代嗜热栖热放线菌的5S rRNA，而真核生物的5S rRNA产生的核糖体亚基生物学活性降低。根据我们的结果，我们提出在50S核糖体亚基组装过程中，5S rRNA的42-47位核苷酸与23S rRNA的2603-2608位核苷酸之间存在相互作用。其他关于真核生物5.8S rRNA的实验表明，这些RNA种类即使能掺入重组核糖体，掺入率也非常低。
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