Watanabe Akari, Tamaki Naofumi, Matsuyama Miwa, Kokeguchi Susumu
Department of Oral Health Care and Rehabilitation, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
Department of Preventive Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.
New Microbiol. 2016 Apr;39(2):143-5.
Bacterial contamination in dental unit waterlines (DUWLs) was evaluated by molecular techniques in addition to the conventional culture method. Water samples (n=8) from DUWLs were investigated for heterotrophic bacteria by culture method using R2A agar. The selected bacterial antibiotic-resistance genes and Legionella species-specific 16SrDNA were identified by PCR. The profiles of bacterial contamination in DUWLs were further identified by PCR-DGGE. In this study, no antibiotic-resistant or Legionella genes were detected. Polycyclic aromatic hydrocarbon-degrading bacterium, Novosphingobium sp. was the most prevalent in DUWLs. Conventional PCR and PCR-DGGE were shown to be potentially useful for monitoring of bacterial contamination in DUWLs.
除了传统培养方法外，还通过分子技术评估了牙科设备水线（DUWLs）中的细菌污染情况。使用R2A琼脂通过培养方法对DUWLs的水样（n = 8）进行了异养细菌调查。通过PCR鉴定了选定的细菌抗生素抗性基因和嗜肺军团菌属特异性16SrDNA。通过PCR-DGGE进一步鉴定了DUWLs中的细菌污染情况。在本研究中，未检测到抗生素抗性或嗜肺军团菌基因。多环芳烃降解菌新鞘氨醇菌属在DUWLs中最为普遍。传统PCR和PCR-DGGE被证明对监测DUWLs中的细菌污染可能有用。
