Wessels Hans-Hermann, Imami Koshi, Baltz Alexander G, Kolinski Marcin, Beldovskaya Anastasia, Selbach Matthias, Small Stephen, Ohler Uwe, Landthaler Markus
Berlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine, 13125 Berlin, Germany;
Department of Biology, New York University, New York, New York 10003, USA;
Genome Res. 2016 Jul;26(7):1000-9. doi: 10.1101/gr.200386.115. Epub 2016 Apr 28.
Early embryogenesis is characterized by the maternal to zygotic transition (MZT), in which maternally deposited messenger RNAs are degraded while zygotic transcription begins. Before the MZT, post-transcriptional gene regulation by RNA-binding proteins (RBPs) is the dominant force in embryo patterning. We used two mRNA interactome capture methods to identify RBPs bound to polyadenylated transcripts within the first 2 h of Drosophila melanogaster embryogenesis. We identified a high-confidence set of 476 putative RBPs and confirmed RNA-binding activities for most of 24 tested candidates. Most proteins in the interactome are known RBPs or harbor canonical RBP features, but 99 exhibited previously uncharacterized RNA-binding activity. mRNA-bound RBPs and TFs exhibit distinct expression dynamics, in which the newly identified RBPs dominate the first 2 h of embryonic development. Integrating our resource with in situ hybridization data from existing databases showed that mRNAs encoding RBPs are enriched in posterior regions of the early embryo, suggesting their general importance in posterior patterning and germ cell maturation.
早期胚胎发育的特征是母源到合子的转变（MZT），在此过程中，母源沉积的信使核糖核酸（mRNA）被降解，同时合子转录开始。在MZT之前，RNA结合蛋白（RBP）介导的转录后基因调控是胚胎模式形成中的主导力量。我们使用了两种mRNA相互作用组捕获方法，来鉴定在黑腹果蝇胚胎发育的最初2小时内与多聚腺苷酸化转录本结合的RBP。我们鉴定出了一组高度可信的476个假定RBP，并证实了24个测试候选蛋白中的大多数具有RNA结合活性。相互作用组中的大多数蛋白质是已知的RBP或具有典型的RBP特征，但有99个表现出以前未被表征的RNA结合活性。与mRNA结合的RBP和转录因子（TF）表现出不同的表达动态，其中新鉴定出的RBP在胚胎发育的最初2小时占主导地位。将我们的资源与现有数据库中的原位杂交数据相结合表明，编码RBP的mRNA在早期胚胎的后部区域富集，这表明它们在后部模式形成和生殖细胞成熟中具有普遍重要性。
