Biomolecular Sciences Research Complex, EaStCHEM School of Chemistry, University of St Andrews, Fife KY16 9ST, UK.
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
Analyst. 2016 Jun 20;141(13):3997-4000. doi: 10.1039/c6an00795c.
We have constructed a 'turn-off' and label free bio-sensor using a DNAzyme molecule. This facile bio-sensor is capable of selective detection of mercuric ions with a high sensitivity and satisfactory dynamic range. More importantly, it is able to generate both fluorescent and colourimetric signals for detection. This dual-channel bio-sensor is expected to afford high detection confidence and overcome false-positive readout especially when assaying complex biological samples.
我们构建了一种“关闭”和无标记的生物传感器，使用了一种 DNA 酶分子。这种简单的生物传感器能够选择性地检测汞离子，具有高灵敏度和令人满意的动态范围。更重要的是，它能够产生荧光和比色信号进行检测。这种双通道生物传感器有望提供高检测置信度，并克服假阳性读数，特别是在分析复杂的生物样本时。
