Pitondo-Silva André, Gonçalves Guilherme Bartolomeu, Stehling Eliana Guedes
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto - Universidade de São Paulo (USP), Ribeirão Preto, Brazil.
APMIS. 2016 Aug;124(8):681-8. doi: 10.1111/apm.12553. Epub 2016 May 20.
Pseudomonas aeruginosa is an opportunistic pathogen, which can have several virulence factors that confer on it the ability to cause severe, acute and chronic infections. Thus, the simultaneous occurrence of resistance to antibiotics and heavy metals associated with the presence of virulence genes is a potential threat to human health and environmental balance. This study aimed to investigate the resistance profile to heavy metals and the correlation of this phenotype of resistance to antimicrobials and to investigate the pathogenic potential of 46 P. aeruginosa isolates obtained from the soil of five Brazilian regions. The bacteria were evaluating for antimicrobial and heavy metal resistance, as well as the presence of plasmids and virulence genes. The isolates showed resistance to four different antibiotics and the majority (n = 44) had resistance to aztreonam or ticarcillin, furthermore, 32 isolates showed concomitant resistance to both of these antibiotics. A high prevalence of virulence genes was found, which highlights the pathogenic potential of the studied environmental isolates. Moreover, a high frequency of heavy metal resistance genes was also detected, however, the phenotypic results indicated that other genes and/or mechanisms should be related to heavy metal resistance.
铜绿假单胞菌是一种机会致病菌，它可拥有多种毒力因子，使其具备引发严重急性和慢性感染的能力。因此，对抗生素和重金属的耐药性与毒力基因的存在同时出现，对人类健康和环境平衡构成了潜在威胁。本研究旨在调查46株从巴西五个地区土壤中分离得到的铜绿假单胞菌对重金属的耐药情况以及这种耐药表型与抗菌药物耐药性的相关性，并研究其致病潜力。对这些细菌进行了抗菌和重金属耐药性评估，以及质粒和毒力基因的检测。分离株对四种不同抗生素呈现耐药性，大多数（n = 44）对氨曲南或替卡西林耐药，此外，32株分离株对这两种抗生素均呈现同时耐药。发现毒力基因的高流行率，这凸显了所研究的环境分离株的致病潜力。此外，还检测到重金属耐药基因的高频率存在，然而，表型结果表明其他基因和/或机制应与重金属耐药性有关。
